C57BL/6NCya-Ddr1em1flox/Cya
Common Name:
Ddr1-flox
Product ID:
S-CKO-01503
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ddr1-flox
Strain ID
CKOCMP-12305-Ddr1-B6N-VA
Gene Name
Product ID
S-CKO-01503
Gene Alias
6030432F18; CD167a; Cak; Nep; PTK3A
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ddr1em1flox/Cya mice (Catalog S-CKO-01503) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000119825
NCBI RefSeq
NM_001198833
Target Region
Exon 5~6
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Ddr1, short for Discoidin domain receptor 1, is a collagen-activated receptor tyrosine kinase. It plays a crucial role in regulating various vital processes such as cell differentiation, proliferation, adhesion, migration, invasion, and matrix remodeling [1]. It is involved in multiple signaling pathways, for example, the DDR1-NF-κB-p62-NRF2 pathway in pancreatic cancer [3]. Given its roles in numerous biological processes, genetic models like gene knockout (KO) or conditional knockout (CKO) mouse models are valuable for studying its functions.
In tumor-related studies, ablation of Ddr1 in tumors promotes intratumoral penetration of T cells and obliterates tumor growth in mouse models of triple-negative breast cancer (TNBC), indicating its role in instigating immune exclusion [2]. In pancreatic ductal adenocarcinoma (PDAC), matrix-metalloprotease-cleaved Col I activates DDR1-NF-κB-p62-NRF2 signaling to promote tumor growth, while intact Col I triggers DDR1 degradation and restrains growth [3]. In hepatocellular carcinoma (HCC), collagen I-DDR1 signaling promotes cancer cell stemness [4]. In kidney-related research, Ddr1-null mice have reduced acute tubular injury, inflammation, and tubulointerstitial fibrosis after ischemia/reperfusion-induced acute kidney injury, suggesting DDR1 contributes to kidney inflammation and fibrosis [5].
In conclusion, Ddr1 is essential in regulating multiple biological processes. Studies using Ddr1 KO/CKO mouse models have revealed its significant roles in various disease areas, including cancer and kidney diseases. These models have provided valuable insights into the functions of Ddr1, helping to understand the mechanisms of disease occurrence and potentially offering new therapeutic targets.
References:
1. Wu, Donglin, Ding, Zihui, Lu, Tao, Zhang, Feng, Lu, Shuai. 2024. DDR1-targeted therapies: current limitations and future potential. In Drug discovery today, 29, 103975. doi:10.1016/j.drudis.2024.103975. https://pubmed.ncbi.nlm.nih.gov/38580164/
2. Sun, Xiujie, Wu, Bogang, Chiang, Huai-Chin, An, Zhiqiang, Li, Rong. 2021. Tumour DDR1 promotes collagen fibre alignment to instigate immune exclusion. In Nature, 599, 673-678. doi:10.1038/s41586-021-04057-2. https://pubmed.ncbi.nlm.nih.gov/34732895/
3. Su, Hua, Yang, Fei, Fu, Rao, Sun, Beicheng, Karin, Michael. 2022. Collagenolysis-dependent DDR1 signalling dictates pancreatic cancer outcome. In Nature, 610, 366-372. doi:10.1038/s41586-022-05169-z. https://pubmed.ncbi.nlm.nih.gov/36198801/
4. Xiong, Yi-Xiao, Zhang, Xiao-Chao, Zhu, Jing-Han, Zhang, Zhan-Guo, Zhang, Wan-Guang. 2023. Collagen I-DDR1 signaling promotes hepatocellular carcinoma cell stemness via Hippo signaling repression. In Cell death and differentiation, 30, 1648-1665. doi:10.1038/s41418-023-01166-5. https://pubmed.ncbi.nlm.nih.gov/37117273/
5. Borza, Corina M, Bolas, Gema, Bock, Fabian, Zent, Roy, Pozzi, Ambra. 2022. DDR1 contributes to kidney inflammation and fibrosis by promoting the phosphorylation of BCR and STAT3. In JCI insight, 7, . doi:10.1172/jci.insight.150887. https://pubmed.ncbi.nlm.nih.gov/34941574/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen