C57BL/6JCya-Calb1em1flox/Cya
Common Name:
Calb1-flox
Product ID:
S-CKO-01505
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Calb1-flox
Strain ID
CKOCMP-12307-Calb1-B6J-VA
Gene Name
Product ID
S-CKO-01505
Gene Alias
Brain-2; CB; Calb; Calb-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Calb1em1flox/Cya mice (Catalog S-CKO-01505) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029876
NCBI RefSeq
NM_009788
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Calb1, also known as calbindin 1, is a calcium-binding protein. It plays a crucial role in regulating calcium-ion homeostasis, which is essential for numerous cellular processes such as autophagy, cell proliferation, and senescence. It is associated with pathways like the Ca2+-dependent calcineurin/NFAT pathway [3].
In ovarian cancer, knockdown of CALB1 inhibited cell proliferation and colony formation, while overexpression promoted these processes and inhibited senescence by modulating p21 and p27 expression levels. Mechanistically, CALB1 interacts with MDM2 and promotes the interaction between p53 and MDM2, suggesting it may act as an oncogene in ovarian cancer by inhibiting the p53 pathway [1].
In oocytes, knockdown of CALB1 led to reduced calcium-ion levels in the endoplasmic reticulum and mitochondria, resulting in mitochondrial dysfunction and meiotic defects. Overexpression of CALB1 in aging oocytes partially rescued age-related defective phenotypes, indicating its importance in maintaining oocyte quality during aging [2].
In conclusion, Calb1 is vital for maintaining calcium-ion homeostasis, which impacts various biological processes. Its role in ovarian cancer and oocyte aging, as revealed through functional studies including knockdown experiments, highlights its significance in understanding cancer development and age-related reproductive health issues.
References:
1. Cao, Long-Qiao, Wang, Ya-Nan, Liang, Ming, Pan, Mei-Zhou. 2019. CALB1 enhances the interaction between p53 and MDM2, and inhibits the senescence of ovarian cancer cells. In Molecular medicine reports, 19, 5097-5104. doi:10.3892/mmr.2019.10212. https://pubmed.ncbi.nlm.nih.gov/31059057/
2. Han, Yingxue, Du, Zihuan, Wu, Hao, Gao, Shuai, Zeng, Shenming. 2025. CALB1 and RPL23 Are Essential for Maintaining Oocyte Quality and Function During Aging. In Aging cell, 24, e14466. doi:10.1111/acel.14466. https://pubmed.ncbi.nlm.nih.gov/39748132/
3. Raynard, Clotilde, Tessier, Nolwenn, Huna, Anda, Martin, Nadine, Bernard, David. 2022. Expression of the Calcium-Binding Protein CALB1 Is Induced and Controls Intracellular Ca2+ Levels in Senescent Cells. In International journal of molecular sciences, 23, . doi:10.3390/ijms23169376. https://pubmed.ncbi.nlm.nih.gov/36012633/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen