C57BL/6JCya-Cap1em1flox/Cya
Common Name:
Cap1-flox
Product ID:
S-CKO-01523
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cap1-flox
Strain ID
CKOCMP-12331-Cap1-B6J-VA
Gene Name
Product ID
S-CKO-01523
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cap1em1flox/Cya mice (Catalog S-CKO-01523) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000106255
NCBI RefSeq
NM_007598
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Cap1, short for Cyclase-Associated Protein 1, is a ubiquitously expressed protein that plays multiple essential roles. It is highly conserved in evolution and is involved in regulating actin dynamics [1,2,5,6]. Additionally, it participates in signaling pathways related to cAMP dynamics, such as the cAMP-Epac-PKA-Rap1 pathway which is relevant to cell adhesion [3,4]. CAP1 is crucial for various biological processes including cell proliferation, migration, and differentiation [1,2,6]. Genetic models, like knockout models, are valuable for studying its functions.
In Arabidopsis, knockout of Cap1 leads to defects in seed germination, seedling morphology, and seed production due to disrupted pollen tube elongation. However, the cap1-1 mutant shows increased tolerance to salt and mannitol treatment, indicating Cap1 plays a negative role in osmotic stress tolerance in plants [7]. In mouse cerebral cortex, inactivation of Cap1 reduces actin monomers in the cytoplasm, promoting nuclear MRTF translocation and MRTF-SRF activation, suggesting Cap1 represses MRTF-SRF-dependent gene expression [5]. In lung cancer, knockdown of Cap1 in A549 cells inhibits proliferation and migration, and phosphorylated sites S308 and S310 in Cap1 promote lung cancer cell proliferation, migration, and metastasis [1].
In conclusion, Cap1 is a multifunctional protein involved in actin dynamics, cAMP-related signaling, and various biological processes. Gene knockout models in different organisms, such as Arabidopsis and mice, have revealed its role in plant development, osmotic stress response, and mammalian gene regulation and cancer-related processes. Understanding Cap1's functions provides insights into disease mechanisms and potential therapeutic targets, especially in cancer research.
References:
1. Zeng, Jie, Li, Xuan, Liang, Long, Xie, Shuanshuan, Wang, Changhui. 2021. Phosphorylation of CAP1 regulates lung cancer proliferation, migration, and invasion. In Journal of cancer research and clinical oncology, 148, 137-153. doi:10.1007/s00432-021-03819-9. https://pubmed.ncbi.nlm.nih.gov/34636991/
2. Huang, Xiaoli, Chao, Ruihua, Zhang, Yanyang, Liang, Dongli, Wang, Yuan. 2021. CAP1, a target of miR-144/451, negatively regulates erythroid differentiation and enucleation. In Journal of cellular and molecular medicine, 25, 2377-2389. doi:10.1111/jcmm.16067. https://pubmed.ncbi.nlm.nih.gov/33496386/
3. Zhang, Xuefeng, Pizzoni, Alejandro, Hong, Kyoungja, Korkhov, Volodymyr, Altschuler, Daniel L. . CAP1 binds and activates adenylyl cyclase in mammalian cells. In Proceedings of the National Academy of Sciences of the United States of America, 118, . doi:10.1073/pnas.2024576118. https://pubmed.ncbi.nlm.nih.gov/34099549/
4. Ramsey, Auburn, Akana, Lokesh, Miyajima, Erina, Xie, Jennifer Y, Zhou, Guo-Lei. 2023. CAP1 (cyclase-associated protein 1) mediates the cyclic AMP signals that activate Rap1 in stimulating matrix adhesion of colon cancer cells. In Cellular signalling, 104, 110589. doi:10.1016/j.cellsig.2023.110589. https://pubmed.ncbi.nlm.nih.gov/36621727/
5. Khudayberdiev, Sharof, Weiss, Kerstin, Heinze, Anika, Linne, Uwe, Rust, Marco B. 2024. The actin-binding protein CAP1 represses MRTF-SRF-dependent gene expression in mouse cerebral cortex. In Science signaling, 17, eadj0032. doi:10.1126/scisignal.adj0032. https://pubmed.ncbi.nlm.nih.gov/38713765/
6. Hasan, Rokib, Zhou, Guo-Lei. 2019. The Cytoskeletal Protein Cyclase-Associated Protein 1 (CAP1) in Breast Cancer: Context-Dependent Roles in Both the Invasiveness and Proliferation of Cancer Cells and Underlying Cell Signals. In International journal of molecular sciences, 20, . doi:10.3390/ijms20112653. https://pubmed.ncbi.nlm.nih.gov/31151140/
7. Zhang, Qian, Wang, Boya, Kong, Xiangge, Li, Xiaoyi, Wang, Jianmei. 2023. Knockout of cyclase-associated protein CAP1 confers tolerance towards salt and osmotic stress in Arabidopsis. In Journal of plant physiology, 285, 153978. doi:10.1016/j.jplph.2023.153978. https://pubmed.ncbi.nlm.nih.gov/37087999/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen