C57BL/6JCya-Casp1em1flox/Cya
Common Name:
Casp1-flox
Product ID:
S-CKO-01544
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Casp1-flox
Strain ID
CKOCMP-12362-Casp1-B6J-VA
Gene Name
Product ID
S-CKO-01544
Gene Alias
ICE; Il1bc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Casp1em1flox/Cya mice (Catalog S-CKO-01544) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027015
NCBI RefSeq
NM_009807
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Casp1, also known as Caspase-1, is a gene encoding proteins related to cell death. It is involved in pyroptosis, a type of programmed death, and is a key component of the NLRP3 inflammasome which is important for interleukin-1β maturation and inflammatory events. It participates in regulating various biological processes such as the innate immune response [5].
In pancreatic cancer, knockdown of Casp1 inhibits cell viability and migration, and enhances gemcitabine-induced cell death, suggesting its potential as a target for combination therapy [1]. In acute promyelocytic leukemia, all-trans-retinoic acid (ATRA) increases and activates Casp1 to trigger pyroptosis and differentiation of APL cells [2]. In Alzheimer's disease, strongly enhanced active caspase-1 expression is observed, and Casp1 knockout mice carrying Alzheimer's-associated mutations are protected from spatial memory loss and other sequelae, with reduced brain caspase-1 and interleukin-1β activation [3]. In acute myeloid leukemia, high Casp1 expression is associated with poor prognosis, and its inhibition impairs AML cell proliferation [4].
In conclusion, Casp1 plays a significant role in multiple disease conditions including cancer and neurodegenerative diseases. Gene knockout mouse models have been crucial in revealing its functions in these diseases, such as its involvement in cell death, differentiation, and inflammation, providing insights for potential therapeutic strategies.
References:
1. Wang, Xianfeng, Chen, Zheng, Nie, Dingrui, Li, Yangqiu, Zeng, Chengwu. 2023. CASP1 is a target for combination therapy in pancreatic cancer. In European journal of pharmacology, 961, 176175. doi:10.1016/j.ejphar.2023.176175. https://pubmed.ncbi.nlm.nih.gov/37949157/
2. Yu, Xibao, Liu, Xin, Liu, Xuan, Li, Yangqiu, Zeng, Chengwu. 2023. Overexpression of CASP1 triggers acute promyelocytic leukemia cell pyroptosis and differentiation. In European journal of pharmacology, 945, 175614. doi:10.1016/j.ejphar.2023.175614. https://pubmed.ncbi.nlm.nih.gov/36822457/
3. Heneka, Michael T, Kummer, Markus P, Stutz, Andrea, Latz, Eicke, Golenbock, Douglas T. 2012. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice. In Nature, 493, 674-8. doi:10.1038/nature11729. https://pubmed.ncbi.nlm.nih.gov/23254930/
4. Liu, Jing, Zhao, Minyi, Feng, Xiaohui, Zeng, Yunxin, Lin, Dongjun. 2021. Expression and prognosis analyses of CASP1 in acute myeloid leukemia. In Aging, 13, 14088-14108. doi:10.18632/aging.203028. https://pubmed.ncbi.nlm.nih.gov/33999861/
5. Kayagaki, Nobuhiko, Warming, Søren, Lamkanfi, Mohamed, Roose-Girma, Merone, Dixit, Vishva M. 2011. Non-canonical inflammasome activation targets caspase-11. In Nature, 479, 117-21. doi:10.1038/nature10558. https://pubmed.ncbi.nlm.nih.gov/22002608/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen