C57BL/6NCya-Ctnnb1em1flox/Cya
Common Name:
Ctnnb1-flox
Product ID:
S-CKO-01559
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ctnnb1-flox
Strain ID
CKOCMP-12387-Ctnnb1-B6N-VA
Gene Name
Product ID
S-CKO-01559
Gene Alias
Bfc; Catnb; Mesc
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ctnnb1em1flox/Cya mice (Catalog S-CKO-01559) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007130
NCBI RefSeq
NM_007614
Target Region
Exon 7
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Ctnnb1, which encodes β-catenin, is a key regulatory molecule of the Wnt/β-catenin signaling pathway. This pathway is crucial for tissue homeostasis, regulating cell proliferation, differentiation, and function in various biological processes such as organ development [1,2].
Knockout of the intestinal enhancer of Ctnnb1 (ieCtnnb1) led to reduced Ctnnb1 transcription, compromising canonical Wnt signaling in intestinal crypts. Single-cell sequencing showed it altered epithelial compositions and functions of small intestinal crypts. While it hampered epithelial turnovers physiologically, it prevented the occurrence and progression of Wnt/β-catenin-driven colorectal cancers [1].
In conclusion, Ctnnb1 is essential for maintaining normal biological functions through the Wnt/β-catenin signaling pathway. Studies using gene-knockout models, like the ieCtnnb1 knockout mouse model, have revealed its role in intestinal homeostasis and cancer development, providing insights into disease mechanisms and potential therapeutic targets for colorectal cancer [1].
References：
1. Hua, Xiaojiao, Zhao, Chen, Tian, Jianbo, Liu, Ying, Zhou, Yan. 2024. A Ctnnb1 enhancer transcriptionally regulates Wnt signaling dosage to balance homeostasis and tumorigenesis of intestinal epithelia. In eLife, 13, . doi:10.7554/eLife.98238. https://pubmed.ncbi.nlm.nih.gov/39320349/
2. Agaimy, Abbas, Haller, Florian. . CTNNB1 (β-Catenin)-altered Neoplasia: A Review Focusing on Soft Tissue Neoplasms and Parenchymal Lesions of Uncertain Histogenesis. In Advances in anatomic pathology, 23, 1-12. doi:10.1097/PAP.0000000000000104. https://pubmed.ncbi.nlm.nih.gov/26645457/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen