C57BL/6JCya-Ctnnb1em1flox/Cya
Common Name:
Ctnnb1-flox
Product ID:
S-CKO-01560
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ctnnb1-flox
Strain ID
CKOCMP-12387-Ctnnb1-B6J-VA
Gene Name
Product ID
S-CKO-01560
Gene Alias
Bfc; Catnb; Mesc
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ctnnb1em1flox/Cya mice (Catalog S-CKO-01560) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007130
NCBI RefSeq
NM_007614
Target Region
Exon 3
Size of Effective Region
~0.4 kb
Detailed Document
Overview of Gene Research
Ctnnb1, encoding β -catenin, is a key gene in the Wnt/β -catenin signaling pathway. This pathway is pivotal in organ development, tissue homeostasis, and is often dysregulated in cancer [2]. β -catenin is involved in cell-cell adhesion and acts as a transcriptional co-activator, regulating the expression of target genes related to cell cycle regulation [1]. Genetic models, such as knockout (KO) or conditional knockout (CKO) mouse models, are valuable for studying its functions.
Knockout of the ieCtnnb1 enhancer, which is crucial for Ctnnb1 transcription in the intestine, led to a reduction in Ctnnb1 transcription. This compromised the canonical Wnt signaling in intestinal crypts, altered epithelial compositions, and hampered epithelial turnovers in normal conditions. However, it also prevented the occurrence and progression of Wnt/β -catenin-driven colorectal cancers, unveiling an enhancer-dependent mechanism controlling Wnt signaling dosage and intestinal epithelial homeostasis [2].
In conclusion, Ctnnb1 is essential for maintaining normal biological functions through its role in the Wnt/β -catenin signaling pathway. Studies using KO/CKO mouse models, like the ieCtnnb1 knockout, have revealed its significance in intestinal homeostasis and cancer development, providing insights into the mechanisms underlying these processes and potential therapeutic targets for related diseases [2].
References:
1. Ledinek, Živa, Sobočan, Monika, Knez, Jure. 2022. The Role of CTNNB1 in Endometrial Cancer. In Disease markers, 2022, 1442441. doi:10.1155/2022/1442441. https://pubmed.ncbi.nlm.nih.gov/35531470/
2. Hua, Xiaojiao, Zhao, Chen, Tian, Jianbo, Liu, Ying, Zhou, Yan. 2024. A Ctnnb1 enhancer transcriptionally regulates Wnt signaling dosage to balance homeostasis and tumorigenesis of intestinal epithelia. In eLife, 13, . doi:10.7554/eLife.98238. https://pubmed.ncbi.nlm.nih.gov/39320349/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen