C57BL/6JCya-Cbsem1flox/Cya
Common Name:
Cbs-flox
Product ID:
S-CKO-01575
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cbs-flox
Strain ID
CKOCMP-12411-Cbs-B6J-VA
Gene Name
Product ID
S-CKO-01575
Gene Alias
HIP4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cbsem1flox/Cya mice (Catalog S-CKO-01575) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000236853
NCBI RefSeq
NM_144855
Target Region
Exon 3
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Cbs, short for cystathionine β -synthase, encodes an enzyme involved in the transsulfuration pathway, which is crucial for the metabolism of sulfur-containing amino acids. This pathway is essential for the synthesis of cysteine and the production of hydrogen sulfide (H2S), a gaseous signaling molecule with various physiological functions [1,2,3].
Elevated endogenous estrogens stimulate human uterine artery endothelial cell (hUAEC) H2S production by selectively upregulating the expression of Cbs. The human Cbs promoter contains multiple estrogen-responsive elements, and estradiol-17β (E2β) stimulates Cbs transcription via ERα/ERβ-directed gene transcription [3].
In conclusion, Cbs is vital in the transsulfuration pathway for sulfur-containing amino acid metabolism and H2S production. The discovery of estrogen-regulated Cbs expression in hUAECs provides insights into the role of Cbs in vascular-related physiological processes. Understanding Cbs may offer new perspectives on estrogen-related vascular functions and potential therapeutic targets in related diseases.
References：
1. Ereño-Orbea, June, Oyenarte, Iker, Martínez-Cruz, Luis Alfonso. 2013. CBS domains: Ligand binding sites and conformational variability. In Archives of biochemistry and biophysics, 540, 70-81. doi:10.1016/j.abb.2013.10.008. https://pubmed.ncbi.nlm.nih.gov/24161944/
2. Ignoul, Sofie, Eggermont, Jan. . CBS domains: structure, function, and pathology in human proteins. In American journal of physiology. Cell physiology, 289, C1369-78. doi:. https://pubmed.ncbi.nlm.nih.gov/16275737/
3. Bai, Jin, Lechuga, Thomas J, Makhoul, Joshua, Hameed, Afshan, Chen, Dong-Bao. 2023. ERα/ERβ-directed CBS transcription mediates E2β-stimulated hUAEC H2S production. In Journal of molecular endocrinology, 70, . doi:10.1530/JME-22-0175. https://pubmed.ncbi.nlm.nih.gov/36476832/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen