C57BL/6JCya-Cdh11em1flox/Cya
Common Name:
Cdh11-flox
Product ID:
S-CKO-01660
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cdh11-flox
Strain ID
CKOCMP-12552-Cdh11-B6J-VA
Gene Name
Product ID
S-CKO-01660
Gene Alias
Cad11; OSF-4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cdh11em1flox/Cya mice (Catalog S-CKO-01660) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000075190
NCBI RefSeq
NM_009866
Target Region
Exon 4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Cdh11, also known as Cadherin-11 or OB-cadherin, is a classical cadherin and a homophilic, Ca2+-dependent cell adhesion glycoprotein. It mediates cell-cell adhesion, participates in and influences many aspects of human growth and development, and is involved in regulating stem cell fate decisions [1,6].
In disease research, CDH11 has diverse roles. In pancreatic ductal adenocarcinomas (PDACs), knockout of CDH11 in KPC mice prolonged survival, reduced stromal activation, extracellular matrix deposition, and immunosuppression, and increased the response to gemcitabine [5]. In liver fibrosis, CDH11-deficient mice showed decreased hepatic stellate cell (HSC) activation in vitro and less liver fibrogenesis in response to CCl4-induced chronic damage, indicating that CDH11 promotes liver fibrosis by activating HSCs [3].
In tongue squamous cell carcinoma (TSCC), overexpression of CDH11 increased adhesion with human oral epithelial cells and decreased transcellular migration ability, suggesting it acts as a tumor suppressor [4]. In head and neck squamous cell carcinoma (HNSCC), siRNA-mediated knockdown of CDH11 enhanced proliferation and invasion, also suggesting its role as a tumor suppressor [8]. In osteosarcoma, the expression level of CDH11 correlated with patient survival, with lower levels in metastatic cell lines compared to primary and normal osteoblast cell lines [9].
In stricturing Crohn's disease, CDH11 was up-regulated in strictures, and its profibrotic function was validated using in vitro gain-and loss-of-function experiments and animal models [7]. In breast cancer, YTHDF1 promotes osteolytic bone metastasis by inducing CDH11 translation [2].
In conclusion, Cdh11 is a crucial gene in cell-cell adhesion and stem cell fate regulation. Gene knockout and knockdown models in mice and cell lines have revealed its complex roles in various diseases, including promoting or suppressing tumor growth and metastasis, and contributing to fibrotic diseases. Understanding Cdh11's functions provides potential targets for disease diagnosis and treatment in areas such as oncology, fibrosis-related diseases, and inflammatory bowel diseases.
References：
1. Zhang, Gaoxiang, Wang, Xi, Zhang, Qingguo. 2023. Cdh11: Roles in different diseases and potential value in disease diagnosis and treatment. In Biochemistry and biophysics reports, 36, 101576. doi:10.1016/j.bbrep.2023.101576. https://pubmed.ncbi.nlm.nih.gov/38034129/
2. Wang, Shuoer, Xu, Lun, Wang, Dongliang, Song, Shaoli, Yan, Wangjun. 2024. YTHDF1 promotes the osteolytic bone metastasis of breast cancer via inducing EZH2 and CDH11 translation. In Cancer letters, 597, 217047. doi:10.1016/j.canlet.2024.217047. https://pubmed.ncbi.nlm.nih.gov/38871245/
3. Ruan, Wanyuan, Pan, Runsang, Shen, Xiaoxu, Nie, Yingjie, Wu, Yayun. 2018. CDH11 promotes liver fibrosis via activation of hepatic stellate cells. In Biochemical and biophysical research communications, 508, 543-549. doi:10.1016/j.bbrc.2018.11.153. https://pubmed.ncbi.nlm.nih.gov/30509494/
4. Zheng, Bi-Tan, Li, Qing-Ling, Lan, Ting, Zheng, Da-Li, Su, Bo-Hua. 2021. CDH11 Regulates Adhesion and Transcellular Migration of Tongue Squamous Cell Carcinoma. In OncoTargets and therapy, 14, 4211-4222. doi:10.2147/OTT.S298614. https://pubmed.ncbi.nlm.nih.gov/34295163/
5. Peran, Ivana, Dakshanamurthy, Sivanesan, McCoy, Matthew D, Assefnia, Shahin, Byers, Stephen W. 2020. Cadherin 11 Promotes Immunosuppression and Extracellular Matrix Deposition to Support Growth of Pancreatic Tumors and Resistance to Gemcitabine in Mice. In Gastroenterology, 160, 1359-1372.e13. doi:10.1053/j.gastro.2020.11.044. https://pubmed.ncbi.nlm.nih.gov/33307028/
6. Alimperti, Stella, Andreadis, Stelios T. 2015. CDH2 and CDH11 act as regulators of stem cell fate decisions. In Stem cell research, 14, 270-82. doi:10.1016/j.scr.2015.02.002. https://pubmed.ncbi.nlm.nih.gov/25771201/
7. Mukherjee, Pranab K, Nguyen, Quang Tam, Li, Jiannan, Kravarik, Kellie M, Rieder, Florian. 2023. Stricturing Crohn's Disease Single-Cell RNA Sequencing Reveals Fibroblast Heterogeneity and Intercellular Interactions. In Gastroenterology, 165, 1180-1196. doi:10.1053/j.gastro.2023.07.014. https://pubmed.ncbi.nlm.nih.gov/37507073/
8. Piao, Songlin, Inglehart, Ronald C, Scanlon, Christina Springstead, Banerjee, Rajat, D'Silva, Nisha J. 2016. CDH11 inhibits proliferation and invasion in head and neck cancer. In Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology, 46, 89-97. doi:10.1111/jop.12471. https://pubmed.ncbi.nlm.nih.gov/27397103/
9. Nakajima, Go, Patino-Garcia, Ana, Bruheim, Skjalg, Fodstad, Oystein, Ju, Jingfang. . CDH11 expression is associated with survival in patients with osteosarcoma. In Cancer genomics & proteomics, 5, 37-42. doi:. https://pubmed.ncbi.nlm.nih.gov/18359978/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen