Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6JCya-Chil3em1flox/Cya
Common Name:
Chil3-flox
Product ID:
S-CKO-01720
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Chil3-flox
Strain ID
CKOCMP-12655-Chil3-B6J-VA
Gene Name
Chil3
Product ID
S-CKO-01720
Gene Alias
Chi3l3; ECF-L; Ym1
Background
C57BL/6JCya
NCBI ID
12655
Modification
Conditional knockout
Chromosome
3
Phenotype
MGI:1330860
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chil3em1flox/Cya mice (Catalog S-CKO-01720) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063062
NCBI RefSeq
NM_009892
Target Region
Exon 4~5
Size of Effective Region
~2.4 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Chil3, also known as chitinase-like protein 3 (and CHI3L1 in human), has diverse functions. It is involved in macrophage polarization, which is crucial for immune-related biological processes. In the context of inflammation regulation, Chil3 is associated with the IL-4-STAT6 signaling pathway [3]. It also appears to be related to the CX3CR1-mediated pathway in the context of liver ischemia-reperfusion injury [1].

In a study on liver ischemia-reperfusion injury, M2 macrophage-secreted Chil3 was shown to be an important effector downstream of CX3CR1 deficiency. Addition of CHIL3/CHI3L1 alone improved hepatocellular metabolism and reduced oxygen-glucose deprivation/re-oxygenation-inflicted injuries in cultured mouse and human hepatocytes [1]. In another study, single-cell RNA-sequencing of tumor-bearing mice after different vaccination routes revealed that intratumoral monocytes expressing Chil3 were reduced after intravenous boost with a self-assembling nanoparticle vaccine, and in humans, the Chil3 + monocyte gene signature in CD16-monocytes was associated with worse outcomes [2]. Also, in sepsis-induced acute lung injury, deletion of Padi2 and Padi4 reduced Nlrp3 + proinflammatory macrophages and fostered Chil3 + myeloid cell differentiation into anti-inflammatory macrophages, highlighting the role of Chil3 in inflammation resolution [4].

In conclusion, Chil3 plays essential roles in macrophage-related immune responses, metabolism regulation in the liver, and in the tumor microenvironment. Gene-knockout mouse models, such as the Cx3cr1 KO mice in the liver injury study, have been crucial in revealing these functions. These findings suggest that Chil3 could be a potential target for treating diseases related to liver ischemia-reperfusion injury, certain cancers, and sepsis-induced acute lung injury.

References:
1. Jin, Guanghui, Guo, Na, Liu, Yasong, Yang, Yang, Ou, Jingxing. 2023. 5-aminolevulinate and CHIL3/CHI3L1 treatment amid ischemia aids liver metabolism and reduces ischemia-reperfusion injury. In Theranostics, 13, 4802-4820. doi:10.7150/thno.83163. https://pubmed.ncbi.nlm.nih.gov/37771779/
2. Baharom, Faezzah, Ramirez-Valdez, Ramiro A, Khalilnezhad, Ahad, Ishizuka, Andrew S, Seder, Robert A. 2022. Systemic vaccination induces CD8+ T cells and remodels the tumor microenvironment. In Cell, 185, 4317-4332.e15. doi:10.1016/j.cell.2022.10.006. https://pubmed.ncbi.nlm.nih.gov/36302380/
3. Yang, Tao, Wang, Ruilin, Liu, Honghong, Yang, Xiangdong, Zhao, Yanling. 2020. Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis. In Life sciences, 266, 118903. doi:10.1016/j.lfs.2020.118903. https://pubmed.ncbi.nlm.nih.gov/33340526/
4. Yu, Xin, Song, Yujing, Dong, Tao, Ma, Jianjie, Li, Yongqing. 2024. Loss of PADI2 and PADI4 ameliorates sepsis-induced acute lung injury by suppressing NLRP3+ macrophages. In JCI insight, 9, . doi:10.1172/jci.insight.181686. https://pubmed.ncbi.nlm.nih.gov/39405117/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest