C57BL/6JCya-Chil3em1flox/Cya
Common Name:
Chil3-flox
Product ID:
S-CKO-01720
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chil3-flox
Strain ID
CKOCMP-12655-Chil3-B6J-VA
Gene Name
Product ID
S-CKO-01720
Gene Alias
Chi3l3; ECF-L; Ym1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chil3em1flox/Cya mice (Catalog S-CKO-01720) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063062
NCBI RefSeq
NM_009892
Target Region
Exon 4~5
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Chil3, also known as chitinase-like protein 3 (and CHI3L1 in human), has diverse functions. It is involved in macrophage polarization, which is crucial for immune-related biological processes. In the context of inflammation regulation, Chil3 is associated with the IL-4-STAT6 signaling pathway [3]. It also appears to be related to the CX3CR1-mediated pathway in the context of liver ischemia-reperfusion injury [1].
In a study on liver ischemia-reperfusion injury, M2 macrophage-secreted Chil3 was shown to be an important effector downstream of CX3CR1 deficiency. Addition of CHIL3/CHI3L1 alone improved hepatocellular metabolism and reduced oxygen-glucose deprivation/re-oxygenation-inflicted injuries in cultured mouse and human hepatocytes [1]. In another study, single-cell RNA-sequencing of tumor-bearing mice after different vaccination routes revealed that intratumoral monocytes expressing Chil3 were reduced after intravenous boost with a self-assembling nanoparticle vaccine, and in humans, the Chil3 + monocyte gene signature in CD16-monocytes was associated with worse outcomes [2]. Also, in sepsis-induced acute lung injury, deletion of Padi2 and Padi4 reduced Nlrp3 + proinflammatory macrophages and fostered Chil3 + myeloid cell differentiation into anti-inflammatory macrophages, highlighting the role of Chil3 in inflammation resolution [4].
In conclusion, Chil3 plays essential roles in macrophage-related immune responses, metabolism regulation in the liver, and in the tumor microenvironment. Gene-knockout mouse models, such as the Cx3cr1 KO mice in the liver injury study, have been crucial in revealing these functions. These findings suggest that Chil3 could be a potential target for treating diseases related to liver ischemia-reperfusion injury, certain cancers, and sepsis-induced acute lung injury.
References:
1. Jin, Guanghui, Guo, Na, Liu, Yasong, Yang, Yang, Ou, Jingxing. 2023. 5-aminolevulinate and CHIL3/CHI3L1 treatment amid ischemia aids liver metabolism and reduces ischemia-reperfusion injury. In Theranostics, 13, 4802-4820. doi:10.7150/thno.83163. https://pubmed.ncbi.nlm.nih.gov/37771779/
2. Baharom, Faezzah, Ramirez-Valdez, Ramiro A, Khalilnezhad, Ahad, Ishizuka, Andrew S, Seder, Robert A. 2022. Systemic vaccination induces CD8+ T cells and remodels the tumor microenvironment. In Cell, 185, 4317-4332.e15. doi:10.1016/j.cell.2022.10.006. https://pubmed.ncbi.nlm.nih.gov/36302380/
3. Yang, Tao, Wang, Ruilin, Liu, Honghong, Yang, Xiangdong, Zhao, Yanling. 2020. Berberine regulates macrophage polarization through IL-4-STAT6 signaling pathway in Helicobacter pylori-induced chronic atrophic gastritis. In Life sciences, 266, 118903. doi:10.1016/j.lfs.2020.118903. https://pubmed.ncbi.nlm.nih.gov/33340526/
4. Yu, Xin, Song, Yujing, Dong, Tao, Ma, Jianjie, Li, Yongqing. 2024. Loss of PADI2 and PADI4 ameliorates sepsis-induced acute lung injury by suppressing NLRP3+ macrophages. In JCI insight, 9, . doi:10.1172/jci.insight.181686. https://pubmed.ncbi.nlm.nih.gov/39405117/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen