C57BL/6JCya-Chrm3em1flox/Cya
Common Name:
Chrm3-flox
Product ID:
S-CKO-01727
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chrm3-flox
Strain ID
CKOCMP-12671-Chrm3-B6J-VA
Gene Name
Product ID
S-CKO-01727
Gene Alias
Chrm-3; M3; M3R
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chrm3em1flox/Cya mice (Catalog S-CKO-01727) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000187510
NCBI RefSeq
NM_033269
Target Region
Exon 5
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Chrm3, encoding the muscarinic acetylcholine receptor M3, is a key gene involved in multiple physiological processes. The M3 receptor is one of the well-characterized receptors of pancreatic β cells and is known to regulate insulin secretion. It is also associated with various signaling pathways, such as those related to calcium regulation [3,4,5].
In disease-related studies, in a severe acute pancreatitis mice model, Chrm3 was upregulated in pancreatitis. Reduction of Chrm3 decreased the pathological lesion of severe acute pancreatitis and reduced amylase activities in serum, suggesting that Chrm3 can suppress acinar cells necrosis, at least in part by stabilizing caspase-8 [3]. In addition, in L-arginine-induced severe acute pancreatitis, CHRM3 activation was found to promote necrosis through the MAPK-p38/miR-31-5p/RIP3 axis [5].
In glioblastoma, high levels of CHRM3 correlated with poor prognosis. Knockdown of CHRM3 inhibited GBM cell growth and invasion, and suppression of CHRM3 in an orthotopic GBM animal model significantly prolonged survival time [2].
In colon cancer, the proliferative effects of bile acids on colon epithelium are through interaction with muscarinic-3 receptors, and CHRM3-associated miRNAs might play a role in bile acid-induced proliferation of H508 colon cancer cells [1].
In castration-resistant prostate cancer, CHRM3-mediated focal adhesion kinase/YES-associated protein (YAP) signalling is essential for cell growth, and miR-15b-5p can inhibit this growth by targeting CHRM3 [6].
In conclusion, Chrm3 plays diverse and crucial roles in various biological processes and disease conditions. Gene-knockout and other loss-of-function experiments, especially in mouse models, have been instrumental in revealing its functions in diseases like pancreatitis, glioblastoma, colon cancer, and prostate cancer. Understanding the role of Chrm3 provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Aktan, Çağdaş, Tekin, Fatih, Oruç, Nevin, Özütemiz, Ömer. . CHRM3-Associated miRNAs May Play a Role in Bile Acid-Induced Proliferation of H508 Colon Cancer Cells. In The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology, 34, 298-307. doi:10.5152/tjg.2022.22605. https://pubmed.ncbi.nlm.nih.gov/36919835/
2. Zhang, Bin, Zhao, Jianyi, Wang, Yongzhi, Zhang, Junchen, Meng, Wei. 2023. CHRM3 is a novel prognostic factor of poor prognosis and promotes glioblastoma progression via activation of oncogenic invasive growth factors. In Oncology research, 31, 917-927. doi:10.32604/or.2023.030425. https://pubmed.ncbi.nlm.nih.gov/37744266/
3. Huang, Ning, Murtaza, Ghulam, Wang, Lujing, Ma, Ning, Gao, Xu. 2019. Chrm3 protects against acinar cell necrosis by stabilizing caspase-8 expression in severe acute pancreatitis mice model. In Journal of cellular biochemistry, 121, 2618-2631. doi:10.1002/jcb.29483. https://pubmed.ncbi.nlm.nih.gov/31692054/
4. Wan, Jianhua, Wang, Jiale, Wagner, Larry E, Bi, Yan, Ji, Baoan. 2021. Pancreas-specific CHRM3 activation causes pancreatitis in mice. In JCI insight, 6, . doi:10.1172/jci.insight.132585. https://pubmed.ncbi.nlm.nih.gov/34314386/
5. Luan, Jing, Kou, Jiayuan, Huang, Ning, Gao, Xu, Ma, Ning. . Inhibition of CHRM3 Alleviates Necrosis Via the MAPK-p38/miR-31-5p/RIP3 Axis in L-Arginine-Induced Severe Acute Pancreatitis. In Pancreas, 49, 1335-1341. doi:10.1097/MPA.0000000000001684. https://pubmed.ncbi.nlm.nih.gov/33122522/
6. Asai, Shunichi, Goto, Yusuke, Tanigawa, Kengo, Sakamoto, Shinichi, Seki, Naohiko. 2023. MiR-15b-5p inhibits castration-resistant growth of prostate cancer cells by targeting the muscarinic cholinergic receptor CHRM3. In FEBS letters, 597, 1164-1175. doi:10.1002/1873-3468.14598. https://pubmed.ncbi.nlm.nih.gov/36754848/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen