C57BL/6JCya-Clockem1flox/Cya
Common Name:
Clock-flox
Product ID:
S-CKO-01760
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Clock-flox
Strain ID
CKOCMP-12753-Clock-B6J-VA
Gene Name
Product ID
S-CKO-01760
Gene Alias
5330400M04Rik; KAT13D
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Clockem1flox/Cya mice (Catalog S-CKO-01760) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000202651
NCBI RefSeq
NM_001289826
Target Region
Exon 7
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Clock, short for Circadian Locomotor Output Cycles Kaput, is a core circadian clock gene. It is fundamental in regulating the circadian rhythm and mediates multiple biological processes. The circadian mechanism involving Clock and other core circadian genes like BMAL1, PER, and CRY is crucial for the regulation of cellular metabolism, and disruptions may lead to metabolic health issues such as obesity, metabolic syndrome, and type 2 diabetes [1].
In glioblastoma (GBM) mouse and patient-derived xenograft models, CLOCK-directed olfactomedin like 3 (OLFML3) expression causes hypoxia-inducible factor 1-alpha (HIF1α)-mediated transcriptional upregulation of periostin (POSTN), which promotes tumor angiogenesis via activation of the TANK-binding kinase 1 (TBK1) signaling in endothelial cells. Blockade of the CLOCK-directed POSTN-TBK1 axis inhibits tumor progression and angiogenesis, suggesting CLOCK as an actionable therapeutic target for GBM [2].
In human mesenchymal stem cell (hMSC) models, CLOCK expression decreased during aging. CLOCK deficiency accelerated hMSC senescence, while overexpression, even of a transcriptionally inactive form, rejuvenated aged hMSCs. Mechanistically, CLOCK formed complexes with nuclear lamina proteins and KAP1 to maintain heterochromatin architecture. Gene therapy with lentiviral vectors encoding CLOCK promoted cartilage regeneration and attenuated age-related articular degeneration in mice, demonstrating a non-canonical role of CLOCK in stabilizing heterochromatin, promoting tissue regeneration, and mitigating aging-associated chronic diseases [3].
In conclusion, Clock is essential for regulating the circadian rhythm and various biological processes. Model-based research, especially using mouse models in glioblastoma and hMSC studies, has revealed its role in tumor angiogenesis and stem cell-related processes, offering potential therapeutic targets for diseases like GBM and age-related articular degeneration.
References:
1. Schrader, Lauren A, Ronnekleiv-Kelly, Sean M, Hogenesch, John B, Bradfield, Christopher A, Malecki, Kristen Mc. 2024. Circadian disruption, clock genes, and metabolic health. In The Journal of clinical investigation, 134, . doi:10.1172/JCI170998. https://pubmed.ncbi.nlm.nih.gov/39007272/
2. Pang, Lizhi, Dunterman, Madeline, Xuan, Wenjing, Heimberger, Amy B, Chen, Peiwen. 2023. Circadian regulator CLOCK promotes tumor angiogenesis in glioblastoma. In Cell reports, 42, 112127. doi:10.1016/j.celrep.2023.112127. https://pubmed.ncbi.nlm.nih.gov/36795563/
3. Liang, Chuqian, Liu, Zunpeng, Song, Moshi, Qu, Jing, Liu, Guang-Hui. 2020. Stabilization of heterochromatin by CLOCK promotes stem cell rejuvenation and cartilage regeneration. In Cell research, 31, 187-205. doi:10.1038/s41422-020-0385-7. https://pubmed.ncbi.nlm.nih.gov/32737416/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen