C57BL/6JCya-Cxcr3em1flox/Cya
Common Name:
Cxcr3-flox
Product ID:
S-CKO-01766
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cxcr3-flox
Strain ID
CKOCMP-12766-Cxcr3-B6J-VA
Gene Name
Product ID
S-CKO-01766
Gene Alias
Cd183; Cmkar3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cxcr3em1flox/Cya mice (Catalog S-CKO-01766) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000056614
NCBI RefSeq
NM_009910
Target Region
Exon 2
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
CXCR3, a chemokine receptor, binds to three ligands: CXCL9, CXCL10, and CXCL11 [2]. It is highly expressed on innate and adaptive lymphocytes, as well as on various cell subsets in non-immune organs and tissues [4]. CXCR3 plays a central role in the generation of cellular inflammation, involved in protective responses to pathogens and pathological conditions related to autoimmunity [4]. It also participates in Th1-type response, and its ligands' production is strongly induced by interferon gamma [3].
Genetic ablation of CXCR3 in Treg cells in multiple cancer models disrupted DC1-Treg cell interactions, increased DC-CD8+ T cell interactions, enhanced tumor antigen-specific cross-presentation by DC1s, and ultimately impaired tumor progression, especially in combination with anti-PD-1 checkpoint blockade immunotherapy [1]. This indicates that CXCR3 is critical for Treg cell accumulation and immune suppression in tumors [1].
In conclusion, CXCR3 is crucial in cellular inflammation, Th1-type response, and disease-related processes. The gene knockout models in cancer research have revealed its role in tumor-immune cell interactions and tumor progression, suggesting it could be a potential therapeutic target in cancer immunotherapy.
References：
1. Moreno Ayala, Mariela A, Campbell, Timothy F, Zhang, Chenyu, Sher, Theo, DuPage, Michel. 2023. CXCR3 expression in regulatory T cells drives interactions with type I dendritic cells in tumors to restrict CD8+ T cell antitumor immunity. In Immunity, 56, 1613-1630.e5. doi:10.1016/j.immuni.2023.06.003. https://pubmed.ncbi.nlm.nih.gov/37392735/
2. Karin, Nathan. 2020. CXCR3 Ligands in Cancer and Autoimmunity, Chemoattraction of Effector T Cells, and Beyond. In Frontiers in immunology, 11, 976. doi:10.3389/fimmu.2020.00976. https://pubmed.ncbi.nlm.nih.gov/32547545/
3. Koper, Olga M, Kamińska, Joanna, Sawicki, Karol, Kemona, Halina. . CXCL9, CXCL10, CXCL11, and their receptor (CXCR3) in neuroinflammation and neurodegeneration. In Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 27, 849-856. doi:10.17219/acem/68846. https://pubmed.ncbi.nlm.nih.gov/29893515/
4. Rubinstein, Artem, Kudryavtsev, Igor, Arsentieva, Natalia, Isakov, Dmitry, Totolian, Areg A. . CXCR3-Expressing T Cells in Infections and Autoimmunity. In Frontiers in bioscience (Landmark edition), 29, 301. doi:10.31083/j.fbl2908301. https://pubmed.ncbi.nlm.nih.gov/39206903/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen