C57BL/6JCya-Plk3em1flox/Cya
Common Name
Plk3-flox
Product ID
S-CKO-01785
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12795-Plk3-B6J-VA
Status
When using this mouse strain in a publication, please cite “Plk3-flox Mouse (Catalog S-CKO-01785) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Plk3-flox
Strain ID
CKOCMP-12795-Plk3-B6J-VA
Gene Name
Product ID
S-CKO-01785
Gene Alias
Cnk, Fnk, PRK, PLK-3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 4 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000076859
NCBI Transcript ID
NM_013807
Target Region
Exon 1~5
Size of Effective Region
~3.2 kb
Overview of Gene Research
Plk3, a member of the polo-like kinases (Plks) family, is a serine/threonine protein kinase. Plks are key regulators of the cell cycle, especially in the G2 phase and mitosis, and Plk3, along with Plk2, seems to have additional functions during G1, S, and G2 phases [2]. It is involved in multiple cellular processes such as the control of the cell cycle, apoptosis, and response to various stresses [3].
In cancer-related studies, high PLK3 expression was found to be associated with higher tumor stage and unfavorable prognosis in colorectal cancer patients, with a PLK3-positive rate ≥50% showing a strong correlation with shortened disease-free and overall survival [6]. In glioblastoma, PLK3 promoted the proneural-mesenchymal transition and tumor proliferation, and targeting it could decrease tumor growth and radioresistance [4]. In breast cancer, LINC00115 acts as a scaffold to link SETDB1 and PLK3, enhancing SETDB1 methylation of PLK3, which affects HIF1α stability and leads to chemoresistance and metastasis [1]. In renal ischemia-reperfusion injury, PLK3 was involved in oxidative stress-induced DNA damage and renal tubular epithelial cell apoptosis, and its inhibition attenuated apoptosis by blocking the ATM/P53-mediated DNA damage response [7]. In the context of influenza virus, PLK3 interacted with the viral nucleoprotein of swine influenza virus, promoting viral replication [5].
In conclusion, Plk3 is crucial in cell cycle regulation, stress response, and apoptosis. Its dysregulation is closely associated with various diseases, especially cancers. Studies on Plk3 using different models, though not specifically KO/CKO mouse models in the given references, have provided insights into its role in disease-related biological processes, which may help in developing therapeutic strategies for these diseases.
References：
1. Luo, Fei, Zhang, Mingda, Sun, Bowen, Li, Yanxin, Feng, Haizhong. 2024. LINC00115 promotes chemoresistant breast cancer stem-like cell stemness and metastasis through SETDB1/PLK3/HIF1α signaling. In Molecular cancer, 23, 60. doi:10.1186/s12943-024-01975-3. https://pubmed.ncbi.nlm.nih.gov/38520019/
2. Myer, David L, Bahassi, El Mustapha, Stambrook, Peter J. . The Plk3-Cdc25 circuit. In Oncogene, 24, 299-305. doi:. https://pubmed.ncbi.nlm.nih.gov/15640846/
3. Helmke, C, Becker, S, Strebhardt, K. 2015. The role of Plk3 in oncogenesis. In Oncogene, 35, 135-47. doi:10.1038/onc.2015.105. https://pubmed.ncbi.nlm.nih.gov/25915845/
4. Yu, Shuo, Lv, Lin, Li, Yang, Liu, Tingting, Hu, Tinghua. 2023. PLK3 promotes the proneural-mesenchymal transition in glioblastoma via transcriptional regulation of C5AR1. In Molecular biology reports, 50, 8249-8258. doi:10.1007/s11033-023-08716-7. https://pubmed.ncbi.nlm.nih.gov/37568042/
5. Ren, Caiyue, Chen, Tong, Zhang, Shishuo, Suolang, Sizhu, Zhou, Hongbo. 2023. PLK3 facilitates replication of swine influenza virus by phosphorylating viral NP protein. In Emerging microbes & infections, 12, 2275606. doi:10.1080/22221751.2023.2275606. https://pubmed.ncbi.nlm.nih.gov/37874309/
6. Zeng, Hai, Wang, Qian, Xiang, Ying, Li, Shuang, Zhang, Weijia. 2024. PLK3 is linked with higher tumor stage and unfavorable prognosis in patients with colorectal cancer. In Biomarkers in medicine, 18, 221-230. doi:10.2217/bmm-2023-0591. https://pubmed.ncbi.nlm.nih.gov/38629862/
7. Deng, Weiming, Wei, Xiangling, Xie, Zhenwei, Li, Heng, Na, Ning. 2022. Inhibition of PLK3 Attenuates Tubular Epithelial Cell Apoptosis after Renal Ischemia-Reperfusion Injury by Blocking the ATM/P53-Mediated DNA Damage Response. In Oxidative medicine and cellular longevity, 2022, 4201287. doi:10.1155/2022/4201287. https://pubmed.ncbi.nlm.nih.gov/35783188/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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