C57BL/6JCya-Col3a1em1flox/Cya
Common Name:
Col3a1-flox
Product ID:
S-CKO-01809
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Col3a1-flox
Strain ID
CKOCMP-12825-Col3a1-B6J-VA
Gene Name
Product ID
S-CKO-01809
Gene Alias
Col3a-1; Tsk-2; Tsk2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Col3a1em1flox/Cya mice (Catalog S-CKO-01809) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000087883
NCBI RefSeq
NM_009930
Target Region
Exon 2~4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
COL3A1, encoding the alpha 1 chain of type III collagen, is crucial for extracellular matrix formation. Type III collagen, synthesized as pre-procollagen, is a major structural component in hollow organs like large blood vessels, uterus, and bowel. It also interacts with platelets in blood clotting and signals in wound healing [1].
Inactivation of the murine Col3a1 gene leads to a shorter lifespan in homozygous mutant mice. These mice die prematurely from major artery rupture, mimicking the human vascular type of Ehlers-Danlos syndrome (vEDS) phenotype. Mutations in the COL3A1 gene cause vEDS, the most severe form of Ehlers-Danlos syndrome, where patients often experience sudden death due to large artery rupture [1].
In conclusion, COL3A1 is essential for maintaining the structure and function of tissues with high type III collagen requirements. The study of Col3a1 knockout mouse models has been instrumental in understanding the pathogenesis of vEDS, highlighting the gene's critical role in preventing arterial rupture and maintaining tissue integrity in related disease conditions.
References:
1. Kuivaniemi, Helena, Tromp, Gerard. 2019. Type III collagen (COL3A1): Gene and protein structure, tissue distribution, and associated diseases. In Gene, 707, 151-171. doi:10.1016/j.gene.2019.05.003. https://pubmed.ncbi.nlm.nih.gov/31075413/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen