C57BL/6JCya-Cpeem1flox/Cya
Common Name:
Cpe-flox
Product ID:
S-CKO-01843
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cpe-flox
Strain ID
CKOCMP-12876-Cpe-B6J-VA
Gene Name
Product ID
S-CKO-01843
Gene Alias
CPH; Cph-1; Cph1; NF-alpha1; fat
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cpeem1flox/Cya mice (Catalog S-CKO-01843) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048967
NCBI RefSeq
NM_013494
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Cpe, or Carboxypeptidase E, is a gene with significant implications in cancer research. It has been shown to be involved in promoting tumourigenesis. In cancer, Cpe may act through the activation of the Erk/Wnt pathways, leading to epithelial-mesenchymal transition, which in turn promotes cancer cell proliferation, invasion, and metastasis [1].
In gastric cancer, elevated Cpe expression is associated with poor survival and recurrence prognosis, and it is found in cases with a later clinical stage. Cpe is considered an independent prognostic factor [1].
In hepatocellular carcinoma, exosomes released from cancer cells contain Cpe mRNA and protein, and Cpe is identified as an exosomal bioactive molecule driving the growth and invasion of low-metastatic HCC cells. Cpe-shRNA-loaded exosomes can inhibit malignant tumour cell proliferation via Cyclin D1 and c-MYC suppression [2].
In conclusion, Cpe plays a crucial role in promoting tumourigenesis in various cancers, especially in gastric and liver cancers. The study of Cpe in these cancer models helps to understand the mechanisms of cancer progression, providing potential targets for cancer treatment.
References：
1. Lin, Jiarui, Huang, Chengzhi, Diao, Wenfei, Huang, Shengchao, Wang, Junjiang. 2024. CPE correlates with poor prognosis in gastric cancer by promoting tumourigenesis. In Heliyon, 10, e29901. doi:10.1016/j.heliyon.2024.e29901. https://pubmed.ncbi.nlm.nih.gov/38694095/
2. Hareendran, Sangeetha, Albraidy, Bassam, Yang, Xuyu, Chen, Clark C, Loh, Y Peng. 2022. Exosomal Carboxypeptidase E (CPE) and CPE-shRNA-Loaded Exosomes Regulate Metastatic Phenotype of Tumor Cells. In International journal of molecular sciences, 23, . doi:10.3390/ijms23063113. https://pubmed.ncbi.nlm.nih.gov/35328535/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen