C57BL/6JCya-Crabp2em1flox/Cya
Common Name:
Crabp2-flox
Product ID:
S-CKO-01853
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Crabp2-flox
Strain ID
CKOCMP-12904-Crabp2-B6J-VA
Gene Name
Product ID
S-CKO-01853
Gene Alias
Crabp-2; CrabpII
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crabp2em1flox/Cya mice (Catalog S-CKO-01853) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000005019
NCBI RefSeq
NM_007759
Target Region
Exon 2~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
CRABP2, or Cellular retinoic acid-binding protein 2, is involved in retinoid partitioning between different nuclear receptors. It participates in various biological processes, potentially influencing cell proliferation, motility, apoptosis, and immune-related pathways. It is associated with pathways like Wnt/β-catenin, integrin/FAK/AKT, and epithelial-mesenchymal transition (EMT) [3,4,5,6].
In melanoma, high CRABP2 levels predicted poor prognosis in PD-1 inhibitor-treated patients, and it may regulate immunotherapy by influencing cancer-associated fibroblasts (CAFs) infiltration [1]. In endometrial cancer, high CRABP2 expression was related to serous EC, reduced overall survival, advanced stage and grade [2]. In Hu sheep dermal papilla cells, overexpression of CRABP2 facilitated cell proliferation via the Wnt/β-catenin pathway [3]. In thyroid cancer, CRABP2 was associated with recurrence and promoted invasion via the integrin/FAK/AKT pathway [4]. In lung cancer, it promoted metastasis via HuR and integrin β1/FAK/ERK signaling [5]. In serous ovarian cancer cells, it accelerated EMT by promoting TRIM16 methylation via upregulating EZH2 expression [6]. In prostate cancer, it facilitated cell migration and invasion by activating PI3K/AKT and MAPK signaling pathways through upregulating LAMB3 [7]. In human osteoblast cells, overexpression of CRABP2 inhibited dexamethasone-induced apoptosis [8]. In breast cancer, it was identified as an oncogene promoting tumour cell proliferation, migration, and invasion [9].
In conclusion, CRABP2 is involved in multiple biological functions including cell proliferation, apoptosis, invasion, and immune-related processes. Studies on CRABP2, especially through in vivo models, have revealed its significant roles in various cancers such as melanoma, endometrial, thyroid, lung, ovarian, prostate, and breast cancers, providing potential biomarker and therapeutic target implications for these disease areas.
References:
1. Zeng, Shuangshuang, Chen, X I, Yi, Qiaoli, Yan, Yuanliang, Liu, Shao. 2023. CRABP2 regulates infiltration of cancer-associated fibroblasts and immune response in melanoma. In Oncology research, 32, 261-272. doi:10.32604/or.2023.042345. https://pubmed.ncbi.nlm.nih.gov/38186580/
2. Egan, Donagh, Moran, Bruce, Wilkinson, Michael, le Roux, Carel W, Brennan, Donal J. 2022. CRABP2 - A novel biomarker for high-risk endometrial cancer. In Gynecologic oncology, 167, 314-322. doi:10.1016/j.ygyno.2022.09.020. https://pubmed.ncbi.nlm.nih.gov/36163055/
3. He, Mingliang, Lv, Xiaoyang, Cao, Xiukai, Wang, Shanhe, Sun, Wei. 2023. CRABP2 Promotes the Proliferation of Dermal Papilla Cells via the Wnt/β-Catenin Pathway. In Animals : an open access journal from MDPI, 13, . doi:10.3390/ani13122033. https://pubmed.ncbi.nlm.nih.gov/37370543/
4. Liu, Chien-Liang, Hsu, Yi-Chiung, Kuo, Chi-Yu, Li, Ying-Syuan, Cheng, Shih-Ping. . CRABP2 Is Associated With Thyroid Cancer Recurrence and Promotes Invasion via the Integrin/FAK/AKT Pathway. In Endocrinology, 163, . doi:10.1210/endocr/bqac171. https://pubmed.ncbi.nlm.nih.gov/36240291/
5. Wu, Jun-I, Lin, Yi-Pei, Tseng, Chien-Wei, Chen, Hui-Jane, Wang, Lu-Hai. 2019. Crabp2 Promotes Metastasis of Lung Cancer Cells via HuR and Integrin β1/FAK/ERK Signaling. In Scientific reports, 9, 845. doi:10.1038/s41598-018-37443-4. https://pubmed.ncbi.nlm.nih.gov/30696915/
6. Xie, Tingting, Tan, Minghua, Gao, Yang, Yang, Hong. 2022. CRABP2 accelerates epithelial mesenchymal transition in serous ovarian cancer cells by promoting TRIM16 methylation via upregulating EZH2 expression. In Environmental toxicology, 37, 1957-1967. doi:10.1002/tox.23542. https://pubmed.ncbi.nlm.nih.gov/35442568/
7. Wang, Rui, Liao, Zhaoping, Liu, Chunhua, Cheng, Gang, Li, Sanlian. . CRABP2 promotes cell migration and invasion by activating PI3K/AKT and MAPK signalling pathways via upregulating LAMB3 in prostate cancer. In Journal of biochemistry, 176, 313-324. doi:10.1093/jb/mvae052. https://pubmed.ncbi.nlm.nih.gov/39038078/
8. Zhang, Haiping, Yu, Ziliang, Sun, Farui, Jin, Jin. 2021. Overexpression of CRABP2 inhibits dexamethasone-induced apoptosis in human osteoblast cells. In Journal of orthopaedic surgery and research, 16, 272. doi:10.1186/s13018-021-02386-6. https://pubmed.ncbi.nlm.nih.gov/33879199/
9. Mei, Jie, Cai, Yun, Chen, Lingyan, Pan, Xiang, Zhang, Yan. 2023. The heterogeneity of tumour immune microenvironment revealing the CRABP2/CD69 signature discriminates distinct clinical outcomes in breast cancer. In British journal of cancer, 129, 1645-1657. doi:10.1038/s41416-023-02432-6. https://pubmed.ncbi.nlm.nih.gov/37715025/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen