C57BL/6JCya-Crebbpem1flox/Cya
Common Name:
Crebbp-flox
Product ID:
S-CKO-01858
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Crebbp-flox
Strain ID
CKOCMP-12914-Crebbp-B6J-VA
Gene Name
Product ID
S-CKO-01858
Gene Alias
CBP; CBP/p300; KAT3A; p300/CBP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crebbpem1flox/Cya mice (Catalog S-CKO-01858) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023165
NCBI RefSeq
NM_001025432
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
CREBBP, also known as CREB-binding protein, is a lysine acetyltransferase belonging to the KAT3 family. It modifies histones and non-histone proteins, regulating chromatin accessibility and transcription. It is involved in DNA replication, DNA repair processes such as base excision repair, nucleotide excision repair, and non-homologous end-joining, and also in DNA damage signaling pathways [2].
In diffuse large B-cell lymphoma (DLBCL), CREBBP mutations occur in 8.4% of patients. Mutations or knockdown of CREBBP in B-lymphoma cells inhibit H3K27 acetylation, downregulate FBXW7 expression, activate the NOTCH pathway and downstream CCL2/CSF1 expression. This leads to tumor-associated macrophage polarization to the M2 phenotype and tumor cell proliferation. In B-lymphoma murine models, xenografted tumors with CREBBP mutations have lower H3K27 acetylation, higher M2 macrophage recruitment, and more rapid tumor growth via the FBXW7-NOTCH-CCL2/CSF1 axis [1]. In addition, in B-cell malignancies, CREBBP-deficient cells are selectively vulnerable to AURKA inhibition. Co-targeting CREBBP and AURKA suppresses MYC transcriptionally and post-translationally to induce replication stress and apoptosis, and in vivo studies show that AURKA inhibition is efficacious in delaying tumor progression in CREBBP-deficient cells [3].
In conclusion, CREBBP is crucial for regulating chromatin-related functions and gene expression. Studies using gene-knockout models in B-cell malignancies, especially DLBCL, have revealed its role in tumor-associated macrophage polarization and tumor growth. These findings provide insights into the mechanisms of disease development and potential therapeutic targets for B-cell malignancies.
References:
1. Huang, Yao-Hui, Cai, Kun, Xu, Peng-Peng, Ji, Meng-Meng, Zhao, Wei-Li. 2021. CREBBP/EP300 mutations promoted tumor progression in diffuse large B-cell lymphoma through altering tumor-associated macrophage polarization via FBXW7-NOTCH-CCL2/CSF1 axis. In Signal transduction and targeted therapy, 6, 10. doi:10.1038/s41392-020-00437-8. https://pubmed.ncbi.nlm.nih.gov/33431788/
2. Dutto, Ilaria, Scalera, Claudia, Prosperi, Ennio. 2017. CREBBP and p300 lysine acetyl transferases in the DNA damage response. In Cellular and molecular life sciences : CMLS, 75, 1325-1338. doi:10.1007/s00018-017-2717-4. https://pubmed.ncbi.nlm.nih.gov/29170789/
3. Sun, Yichen, Chen, Jianfeng, Hong, Jing Han, Ong, Choon Kiat, Tan, Jing. 2024. Targeting AURKA to induce synthetic lethality in CREBBP-deficient B-cell malignancies via attenuation of MYC expression. In Oncogene, 43, 2172-2183. doi:10.1038/s41388-024-03065-6. https://pubmed.ncbi.nlm.nih.gov/38783101/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen