C57BL/6JCya-Crhbpem1flox/Cya
Common Name
Crhbp-flox
Product ID
S-CKO-01862
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12919-Crhbp-B6J-VA
Status
When using this mouse strain in a publication, please cite “Crhbp-flox Mouse (Catalog S-CKO-01862) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Crhbp-flox
Strain ID
CKOCMP-12919-Crhbp-B6J-VA
Gene Name
Product ID
S-CKO-01862
Gene Alias
CRH-BP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 13 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000221025
NCBI Transcript ID
NM_198408
Target Region
Exon 4
Size of Effective Region
~0.9 kb
Overview of Gene Research
Crhbp, short for corticotropin-releasing hormone-binding protein, plays a crucial role in regulating corticotropin release. It is a key regulator in the neuroendocrine, autonomic, and stress adaptation processes, functioning as a negative feedback regulator within the hypothalamus-pituitary-adrenal (HPA) axis by binding CRH with high affinity to inhibit CRH-induced ACTH secretion from pituitary cells [4].
In tumor-related research, in most tumors, Crhbp is downregulated and is identified as an important factor for predicting the prognosis of cancer patients. Overexpression of Crhbp significantly inhibits cell proliferation of LUAD, LIHC, and KIRC cell lines, and also reduces cell mobility in KIRC and HCC cells, suggesting its anti-tumorigenic properties [1]. In HCC, Crhbp downregulation may be due to p62-mediated autophagy, and Crhbp exerts anti-HCC effects by reducing cyclin B2 expression and dissociating cyclin B2-CDK1 complex [2]. In ccRCC, overexpression of Crhbp suppresses cell proliferation, migration, invasion, and apoptosis, and it may be an effective target for treating ccRCC patients [3].
In conclusion, Crhbp is essential for the normal regulation of the HPA axis. Its dysregulation is closely associated with tumor development, and its downregulation in various tumors is related to poor prognosis. Research on Crhbp provides potential targets for cancer treatment, helping to better understand the mechanisms of tumorigenesis and develop new treatment strategies.
References：
1. Yoo, Wonbeak, Choi, Hyunji, Lee, Jieun, Park, Kyung Chan, Noh, Kyunghee. 2024. CRHBP, a novel multiple cancer biomarker connected with better prognosis and anti-tumorigenicity. In Cancer cell international, 24, 391. doi:10.1186/s12935-024-03562-4. https://pubmed.ncbi.nlm.nih.gov/39614295/
2. Wang, Zhiwei, Li, Mingxing, Liu, Yuanfeng, Liu, Bo, Bai, Tao. 2022. CRHBP is degraded via autophagy and exerts anti-hepatocellular carcinoma effects by reducing cyclin B2 expression and dissociating cyclin B2-CDK1 complex. In Cancer gene therapy, 29, 1217-1227. doi:10.1038/s41417-021-00423-4. https://pubmed.ncbi.nlm.nih.gov/35082401/
3. Yang, Kang, Xiao, Yusha, Xu, Tao, Luo, Pengcheng, Cheng, Fan. 2019. Integrative analysis reveals CRHBP inhibits renal cell carcinoma progression by regulating inflammation and apoptosis. In Cancer gene therapy, 27, 607-618. doi:10.1038/s41417-019-0138-2. https://pubmed.ncbi.nlm.nih.gov/31570754/
4. Wan, Yiping, Zhang, Zheng, Lin, Dongliang, Li, Juan, Wang, Yajun. 2022. Characterization of CRH-Binding Protein (CRHBP) in Chickens: Molecular Cloning, Tissue Distribution and Investigation of Its Role as a Negative Feedback Regulator within the Hypothalamus-Pituitary-Adrenal Axis. In Genes, 13, . doi:10.3390/genes13101680. https://pubmed.ncbi.nlm.nih.gov/36292565/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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