C57BL/6JCya-Csf2em1flox/Cya
Common Name:
Csf2-flox
Product ID:
S-CKO-01905
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Csf2-flox
Strain ID
CKOCMP-12981-Csf2-B6J-VB
Gene Name
Product ID
S-CKO-01905
Gene Alias
CSF; Csfgm; GMCSF; Gm-CSf; MGI-IGM
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Csf2em1flox/Cya mice (Catalog S-CKO-01905) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019060
NCBI RefSeq
NM_009969
Target Region
Exon 3~4
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Csf2, also known as colony-stimulating factor 2 or granulocyte-macrophage colony-stimulating factor (GM-CSF), is a potent cytokine. It stimulates myeloid cells such as dendritic cells and macrophages, and is involved in many biological processes including inflammation regulation and cell growth [3]. CSF2 is associated with multiple pathways such as the Akt/Mtor, p-Stat5, and Notch pathways, and plays a role in various diseases [2,4,5]. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.
In ANCA-induced glomerulonephritis, CCR2 -/- chimeric mice (lacking classical monocytes) and CSF2rb -/- chimeric mice (deficient in CSF2 receptor subunit beta) were protected from necrotizing crescentic glomerulonephritis, indicating that CSF2-dependent monocyte activation is crucial for the disease [1]. In sepsis-induced acute kidney injury, intraperitoneal injection of a Csf2-neutralizing antibody after cecal ligation and puncture aggravated kidney injury, while recombinant mouse Csf2 protein rescued mice, suggesting Csf2 promotes alternative macrophage transition and is beneficial for kidney repair [4]. In gliomas, knockdown of CSF2 in glioma cells reduced microglia-dependent glioma invasion, and CSF2-depleted gliomas were smaller with less microglia and macrophage infiltration, showing that tumour-derived CSF2 controls myeloid cell accumulation and glioma progression [6].
In summary, Csf2 is essential in regulating inflammation, macrophage transition, and cancer-related processes. Studies using KO/CKO mouse models have revealed its roles in diseases like ANCA-induced glomerulonephritis, sepsis-induced acute kidney injury, and gliomas. Understanding Csf2's functions through these models provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Rousselle, Anthony, Sonnemann, Janis, Amann, Kerstin, Kettritz, Ralph, Schreiber, Adrian. 2022. CSF2-dependent monocyte education in the pathogenesis of ANCA-induced glomerulonephritis. In Annals of the rheumatic diseases, 81, 1162-1172. doi:10.1136/annrheumdis-2021-221984. https://pubmed.ncbi.nlm.nih.gov/35418479/
2. Yu, Xi, Li, Shenglan, Ke, Shuai, Wang, Huaxin, Wang, Lei. 2024. CSF2 Impairs Nrf2 Signaling through the Akt/Mtor Pathway in the Development of Bladder Cancer. In Journal of Cancer, 15, 3242-3253. doi:10.7150/jca.94343. https://pubmed.ncbi.nlm.nih.gov/38817867/
3. Saita, Kosuke, Moriuchi, Yuta, Iwagawa, Toshiro, Uchida, Kanji, Watanabe, Sumiko. 2022. Roles of CSF2 as a modulator of inflammation during retinal degeneration. In Cytokine, 158, 155996. doi:10.1016/j.cyto.2022.155996. https://pubmed.ncbi.nlm.nih.gov/35988458/
4. Li, Yiming, Zhai, Pan, Zheng, Yawen, Kellum, John A, Peng, Zhiyong. 2020. Csf2 Attenuated Sepsis-Induced Acute Kidney Injury by Promoting Alternative Macrophage Transition. In Frontiers in immunology, 11, 1415. doi:10.3389/fimmu.2020.01415. https://pubmed.ncbi.nlm.nih.gov/32733471/
5. Zhou, Hairong, Gao, Zhenyuan, Wu, Xiao, Wang, Yaping, Zhang, Lu. 2025. CSF2 promotes chemoresistance in colorectal cancer by regulating Notch pathway. In Discover oncology, 16, 495. doi:10.1007/s12672-025-02285-w. https://pubmed.ncbi.nlm.nih.gov/40202620/
6. Sielska, Malgorzata, Przanowski, Piotr, Pasierbińska, Maria, Kettenmann, Helmut, Kaminska, Bozena. 2020. Tumour-derived CSF2/granulocyte macrophage colony stimulating factor controls myeloid cell accumulation and progression of gliomas. In British journal of cancer, 123, 438-448. doi:10.1038/s41416-020-0862-2. https://pubmed.ncbi.nlm.nih.gov/32390004/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen