C57BL/6JCya-Csnk2a1em1flox/Cya
Common Name:
Csnk2a1-flox
Product ID:
S-CKO-01915
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Csnk2a1-flox
Strain ID
CKOCMP-12995-Csnk2a1-B6J-VA
Gene Name
Product ID
S-CKO-01915
Gene Alias
CK II alpha; CK2; Csnk2a1-rs4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Csnk2a1em1flox/Cya mice (Catalog S-CKO-01915) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000099224
NCBI RefSeq
NM_007788
Target Region
Exon 3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Csnk2a1, also known as casein kinase 2 alpha 1, is an important gene involved in multiple biological processes. It is known to participate in various signaling pathways, such as the PI3K-Akt-mTOR signaling pathway [2,5]. CSNK2A1 has been shown to be involved in tumorigenesis by enhancing several oncogenic signaling pathways in various cancers [2].
In pancreatic ductal adenocarcinoma (PDAC), targeted silencing of CSNK2A1 expression significantly enhances sensitivity of PDAC cells to gemcitabine treatment, as CSNK2A1 acts as a key regulator conferring gemcitabine resistance via inducing autophagy [1]. In gastric cancer, silencing CSNK2A1 expression effectively inhibits its oncogenic function, and CSNK2A1 promotes gastric cancer invasion through the PI3K-Akt-mTOR signaling pathway [2]. In cervical cancer, CSNK2A1-mediated phosphorylation of HMGA2 modulates cisplatin resistance, and inhibition of CSNK2A1 can sensitize tumor cells to cisplatin [3]. In osteosarcoma, knockdown of CSNK2A1 potentiates the cytotoxic effects of doxorubicin, as CSNK2A1 induces resistance to doxorubicin through SIRT6-mediated activation of the DNA damage repair pathway [4].
In conclusion, CSNK2A1 plays crucial roles in cancer-related biological processes, especially in drug resistance in various cancers such as PDAC, gastric cancer, cervical cancer, and osteosarcoma. The findings from loss-of-function experiments in these contexts help to understand its functions and suggest potential therapeutic strategies targeting CSNK2A1 for treating these cancers.
References:
1. Liu, Zhi-De, Shi, Yin-Hao, Xu, Qiong-Cong, Zhao, Wei, Yin, Xiao-Yu. 2024. CSNK2A1 confers gemcitabine resistance to pancreatic ductal adenocarcinoma via inducing autophagy. In Cancer letters, 585, 216640. doi:10.1016/j.canlet.2024.216640. https://pubmed.ncbi.nlm.nih.gov/38290659/
2. Jiang, Chao, Ma, Zhenghong, Zhang, Guoan, Du, Qin, Wang, Weibo. 2019. CSNK2A1 Promotes Gastric Cancer Invasion Through the PI3K-Akt-mTOR Signaling Pathway. In Cancer management and research, 11, 10135-10143. doi:10.2147/CMAR.S222620. https://pubmed.ncbi.nlm.nih.gov/31819646/
3. Shi, Zhan, Wu, Ding, Xu, Hao, Yang, Ju, Sun, Xiaoqing. 2021. CSNK2A1-mediated phosphorylation of HMGA2 modulates cisplatin resistance in cervical cancer. In FEBS open bio, 11, 2245-2255. doi:10.1002/2211-5463.13228. https://pubmed.ncbi.nlm.nih.gov/34115920/
4. Hussein, Usama Khamis, Ahmed, Asmaa Gamal, Song, Yiping, Kim, Jung Ryul, Jang, Kyu Yun. 2021. CK2α/CSNK2A1 Induces Resistance to Doxorubicin through SIRT6-Mediated Activation of the DNA Damage Repair Pathway. In Cells, 10, . doi:10.3390/cells10071770. https://pubmed.ncbi.nlm.nih.gov/34359939/
5. Liu, Jilong. 2023. P300 increases CSNK2A1 expression which accelerates colorectal cancer progression through activation of the PI3K-AKT-mTOR axis. In Experimental cell research, 430, 113694. doi:10.1016/j.yexcr.2023.113694. https://pubmed.ncbi.nlm.nih.gov/37391010/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen