C57BL/6JCya-Ctsbem1flox/Cya
Common Name:
Ctsb-flox
Product ID:
S-CKO-01934
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ctsb-flox
Strain ID
CKOCMP-13030-Ctsb-B6J-VA
Gene Name
Product ID
S-CKO-01934
Gene Alias
CB
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ctsbem1flox/Cya mice (Catalog S-CKO-01934) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006235
NCBI RefSeq
NM_007798
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Ctsb, also known as Cathepsin B, is a cysteine protease. It is mainly located in lysosomes and participates in various cellular processes such as protein degradation and antigen processing. It is involved in multiple pathways including the mitochondrial apoptosis pathway, mitophagy-ROS-NLRP3 pathway, and is related to the function of osteoclasts, immune cells infiltration, and autophagy [1-10].
In sepsis-induced acute kidney injury (S-AKI), Ctsb activation via lysosomal membrane permeabilization (LMP) promotes apoptosis through the mitochondrial apoptosis pathway, and its inhibition may be a new treatment strategy [1]. In high-fat-diet-induced hepatic pyroptosis, apigenin alleviates pyroptosis by reducing Ctsb release caused by LMP through the mitophagy-ROS-CTSB-NLRP3 pathway [2]. CST6 protein and peptides can inhibit breast cancer bone metastasis by suppressing Ctsb activity and osteoclastogenesis [3]. In AML, knockdown of Ctsb in HL-60 cells inhibits cell proliferation and tumorigenesis, and may serve as an independent prognostic factor [4]. In gliomas, Ctsb is a negative prognostic biomarker related to immune cells infiltration and immunosuppression [5]. In late middle-aged adults at risk for Alzheimer's disease, aerobic exercise training increases plasma Ctsb, and changes in Ctsb correlate with cognitive performance [6]. In mice, running-induced systemic Ctsb secretion is associated with memory function, and Ctsb knockout mice do not show exercise-enhanced adult hippocampal neurogenesis and spatial memory function [7]. In necroptosis, MLKL polymerization-induced LMP leads to Ctsb release and cell death, and chemical inhibition or knockdown of Ctsb protects cells from necroptosis [8]. Melittin treatment suppresses malignant NSCLC progression through enhancing Ctsb-mediated hyperautophagy [9].
In conclusion, Ctsb is involved in multiple biological processes and diseases. Studies using gene knockout or knockdown models have revealed its role in apoptosis, pyroptosis, cancer metastasis, cell proliferation, cognitive function, and necroptosis. These findings provide insights into potential therapeutic strategies for related diseases.
References：
1. Wang, Yuting, Xi, Wenjie, Zhang, Xinyi, Zheng, Xiaoming, Chi, Xinjin. 2023. CTSB promotes sepsis-induced acute kidney injury through activating mitochondrial apoptosis pathway. In Frontiers in immunology, 13, 1053754. doi:10.3389/fimmu.2022.1053754. https://pubmed.ncbi.nlm.nih.gov/36713420/
2. Meng, Zhuoqun, Gao, Min, Wang, Chunyun, Zhang, DuoDuo, Lu, Jing. 2023. Apigenin Alleviated High-Fat-Diet-Induced Hepatic Pyroptosis by Mitophagy-ROS-CTSB-NLRP3 Pathway in Mice and AML12 Cells. In Journal of agricultural and food chemistry, 71, 7032-7045. doi:10.1021/acs.jafc.2c07581. https://pubmed.ncbi.nlm.nih.gov/37141464/
3. Li, Xiaoxun, Liang, Yajun, Lian, Cheng, Liu, Tong, Hu, Guohong. 2021. CST6 protein and peptides inhibit breast cancer bone metastasis by suppressing CTSB activity and osteoclastogenesis. In Theranostics, 11, 9821-9832. doi:10.7150/thno.62187. https://pubmed.ncbi.nlm.nih.gov/34815788/
4. Peng, Sida, Yang, Qingqing, Li, Huan, Hu, Pan, Zhang, Nana. 2021. CTSB Knockdown Inhibits Proliferation and Tumorigenesis in HL-60 Cells. In International journal of medical sciences, 18, 1484-1491. doi:10.7150/ijms.54206. https://pubmed.ncbi.nlm.nih.gov/33628106/
5. Ma, Kaiming, Chen, Xin, Liu, Weihai, Yang, Chenlong, Yang, Jun. 2022. CTSB is a negative prognostic biomarker and therapeutic target associated with immune cells infiltration and immunosuppression in gliomas. In Scientific reports, 12, 4295. doi:10.1038/s41598-022-08346-2. https://pubmed.ncbi.nlm.nih.gov/35277559/
6. Gaitán, Julian M, Moon, Hyo Youl, Stremlau, Matthew, Okonkwo, Ozioma C, van Praag, Henriette. 2021. Effects of Aerobic Exercise Training on Systemic Biomarkers and Cognition in Late Middle-Aged Adults at Risk for Alzheimer's Disease. In Frontiers in endocrinology, 12, 660181. doi:10.3389/fendo.2021.660181. https://pubmed.ncbi.nlm.nih.gov/34093436/
7. Moon, Hyo Youl, Becke, Andreas, Berron, David, Duzel, Emrah, van Praag, Henriette. 2016. Running-Induced Systemic Cathepsin B Secretion Is Associated with Memory Function. In Cell metabolism, 24, 332-40. doi:10.1016/j.cmet.2016.05.025. https://pubmed.ncbi.nlm.nih.gov/27345423/
8. Liu, Shuzhen, Perez, Preston, Sun, Xue, Mammadova, Jamila, Wang, Zhigao. 2023. MLKL polymerization-induced lysosomal membrane permeabilization promotes necroptosis. In Cell death and differentiation, 31, 40-52. doi:10.1038/s41418-023-01237-7. https://pubmed.ncbi.nlm.nih.gov/37996483/
9. Wang, Yuhan, Yuan, Tailei, He, Longyue, Huang, Gang, Lu, Changlian. 2024. Melittin treatment suppressed malignant NSCLC progression through enhancing CTSB-mediated hyperautophagy. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 180, 117573. doi:10.1016/j.biopha.2024.117573. https://pubmed.ncbi.nlm.nih.gov/39426282/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen