C57BL/6JCya-Ctsgem1flox/Cya
Common Name:
Ctsg-flox
Product ID:
S-CKO-01938
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ctsg-flox
Strain ID
CKOCMP-13035-Ctsg-B6J-VA
Gene Name
Product ID
S-CKO-01938
Gene Alias
CatG; VSP
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ctsgem1flox/Cya mice (Catalog S-CKO-01938) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015583
NCBI RefSeq
NM_007800
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Ctsg, also known as Cathepsin G, is a member of the serine proteases family. It plays a role in multiple biological processes, such as regulation of immune processes, and is involved in the generation of Angiotensin II within the renin-angiotensin system, which regulates blood pressure and fluid-electrolyte homeostasis [2,3]. It can increase the permeability of vascular endothelial cells and the chemotaxis of inflammatory cells, and may be related to the infiltration of muscle inflammatory cells [4].
In colorectal cancer, Ctsg acts as a tumor suppressor gene. Overexpression of Ctsg suppresses CRC cell progression in vitro and in vivo by negatively regulating the Akt/mTOR/Bcl2 anti-apoptotic signaling pathway, while its suppression promotes CRC development [1].
In Chinese children with type 1 diabetes mellitus, the CTSG rs2236742 allele (C/T) is associated with T1DM, and CTSG expression is significantly higher in patients compared to the control group [2].
In dermatomyositis, the expression and activity of Ctsg are increased, and it may be involved in muscle inflammatory cell infiltration [4]. Also, in the development of chronic postsurgical pain, Ctsg is up-regulated and acts as a pronociceptive mediator, and specific genotypes at polymorphisms rs2070697 or rs2236742 in the CTSG gene are associated with a lower risk of chronic postsurgical pain [5].
In conclusion, Ctsg is involved in various biological functions and disease conditions. Its role as a tumor suppressor in colorectal cancer, its association with type 1 diabetes mellitus, its involvement in dermatomyositis, and its role in chronic postsurgical pain have been revealed through relevant studies. Understanding Ctsg can provide insights into the mechanisms of these diseases and potential therapeutic targets.
References:
1. Chan, Shixin, Wang, Xu, Wang, Zhenglin, Zhang, Huabing, Chen, Wei. 2023. CTSG Suppresses Colorectal Cancer Progression through Negative Regulation of Akt/mTOR/Bcl2 Signaling Pathway. In International journal of biological sciences, 19, 2220-2233. doi:10.7150/ijbs.82000. https://pubmed.ncbi.nlm.nih.gov/37151875/
2. Li, Jiaci, Zhang, Mingying, Zhang, Shuyue, Lv, Ling, Cai, Chunquan. . CTSG polymorphisms in Chinese children with type 1 diabetes mellitus. In Journal of tropical pediatrics, 70, . doi:10.1093/tropej/fmae017. https://pubmed.ncbi.nlm.nih.gov/39122654/
3. Belova, L A. . Angiotensin II-generating enzymes. In Biochemistry. Biokhimiia, 65, 1337-45. doi:. https://pubmed.ncbi.nlm.nih.gov/11173502/
4. Gao, Siming, Zhu, Honglin, Yang, Huan, Li, Qiuxiang, Luo, Hui. 2017. The role and mechanism of cathepsin G in dermatomyositis. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 94, 697-704. doi:10.1016/j.biopha.2017.07.088. https://pubmed.ncbi.nlm.nih.gov/28797985/
5. Liu, Xiaodong, Tian, Yuanyuan, Meng, Zhaoyu, Cheng, Christopher H K, Chan, Matthew T V. . Up-regulation of Cathepsin G in the Development of Chronic Postsurgical Pain: An Experimental and Clinical Genetic Study. In Anesthesiology, 123, 838-50. doi:10.1097/ALN.0000000000000828. https://pubmed.ncbi.nlm.nih.gov/26270939/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen