C57BL/6JCya-Ctswem1flox/Cya
Common Name
Ctsw-flox
Product ID
S-CKO-01944
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-13041-Ctsw-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ctsw-flox Mouse (Catalog S-CKO-01944) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ctsw-flox
Strain ID
CKOCMP-13041-Ctsw-B6J-VA
Gene Name
Product ID
S-CKO-01944
Gene Alias
lymphopain
Background
C57BL/6JCya
Gene Full Name
cathepsin W
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 19 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000025844
NCBI Transcript ID
NM_009985
Target Region
Exon 2
Size of Effective Region
~0.6 kb
Overview of Gene Research
Cathepsin W (CtsW), a cysteine protease, is involved in multiple biological processes. It is crucial for the escape of Influenza A Virus (IAV) from late endosomes, with its proteolytic activity required for the fusion of viral and endosomal membranes. Intracellular CtsW promotes viral entry [3,4]. It may also play a role in the regulation of inflammation, metabolism, autophagy, and apoptosis pathways, as indicated by studies on lumbar disc herniation [7]. Additionally, CtsW expression is associated with immune response and inflammation, with its expression increasing during aging in whole blood [8].
In pancreatic cancer, down-regulation of CtsW is associated with poor prognosis, suggesting its potential as a prognostic and diagnostic marker [1]. In papillary thyroid cancer, circular RNA_14580 interacts with CtsW, and this interaction is involved in tumorigenic processes, highlighting the role of CtsW in modulating the tumor microenvironment [2]. In cutaneous T-cell lymphomas, TCR-γδ+ MF and Berti lymphoma clones show increased expression of CtsW along with other cytotoxic markers [5]. In multiple myeloma, cytotoxic T cell clusters in patients have higher expression of CtsW as a senescence marker [6].
In conclusion, CtsW plays diverse and important roles in various biological processes and disease conditions. Its functions in viral entry, cancer prognosis, and immune-related responses are significant. Studies on CtsW using different models have enhanced our understanding of these roles, providing potential targets for antiviral and cancer therapies, as well as insights into immune-related diseases.
References：
1. Khojasteh-Leylakoohi, Fatemeh, Mohit, Reza, Khalili-Tanha, Nima, Batra, Jyotsna, Avan, Amir. 2023. Down regulation of Cathepsin W is associated with poor prognosis in pancreatic cancer. In Scientific reports, 13, 16678. doi:10.1038/s41598-023-42928-y. https://pubmed.ncbi.nlm.nih.gov/37794108/
2. Wu, Gang, Ding, Chao, Shi, Chen-Lei, Qin, Hua-Dong, Shi, Tie-Feng. 2024. Circular RNA 14580 promotes papillary thyroid cancer progression by interacting with CTSW/fibrosis singling pathway. In International journal of biological macromolecules, 286, 138538. doi:10.1016/j.ijbiomac.2024.138538. https://pubmed.ncbi.nlm.nih.gov/39653218/
3. Edinger, Thomas O, Pohl, Marie O, Yángüez, Emilio, Stertz, Silke. 2015. Cathepsin W Is Required for Escape of Influenza A Virus from Late Endosomes. In mBio, 6, e00297. doi:10.1128/mBio.00297-15. https://pubmed.ncbi.nlm.nih.gov/26060270/
4. Günther, Sira C, Martínez-Romero, Carles, Sempere Borau, Milagros, García-Sastre, Adolfo, Stertz, Silke. 2022. Proteomic Identification of Potential Target Proteins of Cathepsin W for Its Development as a Drug Target for Influenza. In Microbiology spectrum, 10, e0092122. doi:10.1128/spectrum.00921-22. https://pubmed.ncbi.nlm.nih.gov/35867415/
5. Chennareddy, Sumanth, Rindler, Katharina, Ruggiero, John R, Jonak, Constanze, Brunner, Patrick M. . Single-cell RNA sequencing comparison of CD4+, CD8+ and T-cell receptor γδ+ cutaneous T-cell lymphomas reveals subset-specific molecular phenotypes. In The British journal of dermatology, 192, 269-282. doi:10.1093/bjd/ljae313. https://pubmed.ncbi.nlm.nih.gov/39133553/
6. Wan, Yike, Chen, Mengping, Li, Xin, Huang, Honghui, Hou, Jian. 2023. Single-cell RNA sequencing reveals XBP1-SLC38A2 axis as a metabolic regulator in cytotoxic T lymphocytes in multiple myeloma. In Cancer letters, 562, 216171. doi:10.1016/j.canlet.2023.216171. https://pubmed.ncbi.nlm.nih.gov/37054944/
7. Li, Xiaosheng, Li, Shuoqi, Zang, Zhengwu, He, Yinhao. 2022. Yaobishu Regulates Inflammatory, Metabolic, Autophagic, and Apoptosis Pathways to Attenuate Lumbar Disc Herniation. In Oxidative medicine and cellular longevity, 2022, 3861380. doi:10.1155/2022/3861380. https://pubmed.ncbi.nlm.nih.gov/35615578/
8. de Almeida Chuffa, Luiz Gustavo, Freire, Paula Paccielli, Dos Santos Souza, Jeferson, de Oliveira Neto, Mário, Carvalho, Robson Francisco. 2021. Aging whole blood transcriptome reveals candidate genes for SARS-CoV-2-related vascular and immune alterations. In Journal of molecular medicine (Berlin, Germany), 100, 285-301. doi:10.1007/s00109-021-02161-4. https://pubmed.ncbi.nlm.nih.gov/34741638/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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