C57BL/6JCya-Cux2em1flox/Cya
Common Name:
Cux2-flox
Product ID:
S-CKO-01948
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cux2-flox
Strain ID
CKOCMP-13048-Cux2-B6J-VA
Gene Name
Product ID
S-CKO-01948
Gene Alias
1700051K22Rik; Cutl2; Cux-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cux2em1flox/Cya mice (Catalog S-CKO-01948) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086317
NCBI RefSeq
NM_007804
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Cux2, also known as Cut-like homeobox 2, is a transcription factor that plays diverse roles in various biological processes. It controls neuronal proliferation, dendrite branching, and synapse formation, and is involved in limb positioning during development. It also has implications in several signaling pathways, such as those related to tumorigenesis and neurogenesis [4,5,6]. Genetic models, including KO mouse models, are valuable for studying its functions.
In cancer research, loss-of-function experiments show that CUX2 silencing significantly inhibits the proliferation, colony formation, migration, invasion, and promotes apoptosis in papillary thyroid cancer cells. CUX2 may act as an oncogene by inducing epithelial-mesenchymal transition and influencing the phosphorylation of AKT and mTOR in the PI3K-AKT-mTOR pathways [3]. In breast cancer, inhibition of CUX2 suppresses cell malignant phenotypes through the KDM5B/SOX17 axis. CUX2 promotes KDM5B expression, which in turn inhibits SOX17 expression [1]. In gliomas, overexpression of CUX2 represses the proliferative, migrating, and invasive abilities of glioma cells by enhancing ADCY1 transcription [2]. In epilepsy research, Cux2-specific knockout mice show high susceptibility to kainate, increased excitatory cell number in the entorhinal cortex, and enhanced glutamatergic synaptic transmission to the hippocampus, suggesting that CUX2 variants may contribute to temporal lobe epilepsy pathology [4].
In conclusion, Cux2 is crucial for normal development and physiological function. Model-based research, especially KO mouse models, has revealed its significance in cancer and epilepsy. Understanding Cux2's functions can provide insights into the mechanisms of these diseases, potentially leading to new therapeutic strategies.
References:
1. Li, Lili, Zhu, Genbao, Tan, Kemeng, Chen, Jie, Ma, Chengquan. . CUX2/KDM5B/SOX17 Axis Affects the Occurrence and Development of Breast Cancer. In Endocrinology, 163, . doi:10.1210/endocr/bqac110. https://pubmed.ncbi.nlm.nih.gov/35881915/
2. Yao, Guojun, Le, Shihai, Min, Sufang, Cai, Chuanxing, Deng, Ling. 2022. CUX2 prevents the malignant progression of gliomas by enhancing ADCY1 transcription. In Experimental brain research, 240, 3153-3165. doi:10.1007/s00221-022-06481-w. https://pubmed.ncbi.nlm.nih.gov/36242624/
3. Sun, Yihan, Ye, Danrong, Li, Yuefeng, Wang, Ouchen, Zhang, Xiaohua. 2018. CUX2 functions as an oncogene in papillary thyroid cancer. In OncoTargets and therapy, 12, 217-224. doi:10.2147/OTT.S185710. https://pubmed.ncbi.nlm.nih.gov/30636884/
4. Suzuki, Toshimitsu, Tatsukawa, Tetsuya, Sudo, Genki, Moore, Adrian Walton, Yamakawa, Kazuhiro. 2022. CUX2 deficiency causes facilitation of excitatory synaptic transmission onto hippocampus and increased seizure susceptibility to kainate. In Scientific reports, 12, 6505. doi:10.1038/s41598-022-10715-w. https://pubmed.ncbi.nlm.nih.gov/35581205/
5. Ueda, Shogo, Cordeiro, Ingrid Rosenburg, Moriyama, Yuuta, Shirahige, Katsuhiko, Tanaka, Mikiko. 2019. Cux2 refines the forelimb field by controlling expression of Raldh2 and Hox genes. In Biology open, 8, . doi:10.1242/bio.040584. https://pubmed.ncbi.nlm.nih.gov/30651234/
6. Wittmann, Walter, Iulianella, Angelo, Gunhaga, Lena. 2014. Cux2 acts as a critical regulator for neurogenesis in the olfactory epithelium of vertebrates. In Developmental biology, 388, 35-47. doi:10.1016/j.ydbio.2014.01.026. https://pubmed.ncbi.nlm.nih.gov/24512687/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen