C57BL/6JCya-Cyp1a1em1flox/Cya
Common Name:
Cyp1a1-flox
Product ID:
S-CKO-01960
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cyp1a1-flox
Strain ID
CKOCMP-13076-Cyp1a1-B6J-VA
Gene Name
Product ID
S-CKO-01960
Gene Alias
AHH; AHRR; CP11; CYPIA1; P450-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp1a1em1flox/Cya mice (Catalog S-CKO-01960) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000216433
NCBI RefSeq
NM_009992
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Cyp1a1, a cytochrome P450 enzyme, is regulated by the aryl hydrocarbon receptor (AHR) signaling pathway [3]. It plays a crucial role in the metabolism of polycyclic hydrocarbons in the environment and is associated with the metabolism of pro-carcinogenic compounds into highly carcinogenic metabolites [1,3]. Additionally, it has gained significance in drug metabolism, mediating drug-drug interactions, and bioactivation of prodrugs [3]. Gene-targeted mice can serve as a valuable model system to study its in vivo function as a host factor determinant of environmentally-caused cancers in humans [1].
Mice constitutively expressing Cyp1a1 showed increased CYP1A1 enzymatic activity in the skin, leading to exacerbated immune cell activation and skin pathology, similar to Ahr-deficient mice. Inhibition of CYP1A1 enzymatic activity improved skin immunopathology by restoring beneficial AHR signaling, suggesting that dysregulation of the AHR/CYP1A1 axis may be involved in inflammatory skin disease [2]. PM2.5 exposure in mice led to adverse pregnancy outcomes through the KLF9/CYP1A1 transcriptional axis, as PM2.5 stimulated KLF9 to bind to the CYP1A1 promoter region, modulating downstream phenotypes related to oxidative stress and mitochondrial apoptosis in trophoblasts [4].
In conclusion, Cyp1a1 is essential in metabolism, especially of environmental carcinogens, and drug-related processes. Mouse models have revealed its role in inflammatory skin disease and adverse pregnancy outcomes, providing insights into the underlying mechanisms and potential therapeutic targets in these disease areas.
References:
1. Kawajiri, K. . CYP1A1. In IARC scientific publications, , 159-72. doi:. https://pubmed.ncbi.nlm.nih.gov/10493257/
2. Kyoreva, Mariela, Li, Ying, Hoosenally, Mariyah, Barker, Jonathan N, Di Meglio, Paola. 2020. CYP1A1 Enzymatic Activity Influences Skin Inflammation Via Regulation of the AHR Pathway. In The Journal of investigative dermatology, 141, 1553-1563.e3. doi:10.1016/j.jid.2020.11.024. https://pubmed.ncbi.nlm.nih.gov/33385398/
3. Anwar-Mohamed, Anwar, Elbekai, Reem H, El-Kadi, Ayman Os. . Regulation of CYP1A1 by heavy metals and consequences for drug metabolism. In Expert opinion on drug metabolism & toxicology, 5, 501-21. doi:10.1517/17425250902918302. https://pubmed.ncbi.nlm.nih.gov/19416086/
4. Li, Shuxian, Li, Lingbing, Zhang, Changqing, Zhang, Meihua, Wang, Xietong. 2023. PM2.5 leads to adverse pregnancy outcomes by inducing trophoblast oxidative stress and mitochondrial apoptosis via KLF9/CYP1A1 transcriptional axis. In eLife, 12, . doi:10.7554/eLife.85944. https://pubmed.ncbi.nlm.nih.gov/37737576/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen