C57BL/6JCya-Cyp2g1em1flox/Cya
Common Name:
Cyp2g1-flox
Product ID:
S-CKO-01972
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cyp2g1-flox
Strain ID
CKOCMP-13108-Cyp2g1-B6J-VA
Gene Name
Product ID
S-CKO-01972
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp2g1em1flox/Cya mice (Catalog S-CKO-01972) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000040944
NCBI RefSeq
NM_013809
Target Region
Exon 4~5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Cyp2g1 is a cytochrome P450 monooxygenase uniquely expressed in the olfactory mucosa (OM) [1,2,3,4,5,6,7]. It functions as a steroid hydroxylase, metabolizing testosterone and progesterone [3]. The gene is involved in the metabolic activation of xenobiotic compounds like coumarin, acetaminophen (AP), and 2,6-dichlorobenzonitrile (DCBN) [1,5]. Mouse models have been crucial in studying Cyp2g1's function.
In Cyp2g1 -null mice, OM microsomes showed significantly lower activities in testosterone, progesterone metabolism, and coumarin activation [1]. Unexpectedly, Cyp2a5 expression was reduced in the liver, lateral nasal gland (LNG), and kidney [1]. Although systemic clearance and hepatotoxicity of AP were unaffected, the LNG was protected from AP toxicity in Cyp2g1 -null mice [1]. Also, CYP2A5 and CYP2G1 were found to play major roles in mouse OM microsomal metabolic activation of DCBN and AP, with CYP2A5 potentially more important in AP metabolism in OM microsomes and the roles in DCBN metabolism being dose -dependent [5].
In conclusion, Cyp2g1 plays a key role in the metabolism of steroids and xenobiotic compounds in the olfactory mucosa. The use of Cyp2g1 -null mouse models has provided insights into its function, especially in relation to the toxicity of compounds like AP in specific tissues such as the LNG. This contributes to our understanding of the role of Cyp2g1 in maintaining olfactory mucosal health and the potential implications for chemical -induced olfactory system damage [1,5].
References:
1. Zhuo, Xiaoliang, Gu, Jun, Behr, Melissa J, Collins, Doris N, Ding, Xinxin. 2003. Targeted disruption of the olfactory mucosa-specific Cyp2g1 gene: impact on acetaminophen toxicity in the lateral nasal gland, and tissue-selective effects on Cyp2a5 expression. In The Journal of pharmacology and experimental therapeutics, 308, 719-28. doi:. https://pubmed.ncbi.nlm.nih.gov/14610229/
2. Zhuo, X, Schwob, J E, Swiatek, P J, Ding, X. . Mouse cyp2g1 gene: promoter structure and tissue-specific expression of a cyp2g1-lacz fusion gene in transgenic mice. In Archives of biochemistry and biophysics, 391, 127-36. doi:. https://pubmed.ncbi.nlm.nih.gov/11414693/
3. Hua, Z, Zhang, Q Y, Su, T, Lipinskas, T W, Ding, X. . cDNA cloning, heterologous expression, and characterization of mouse CYP2G1, an olfactory-specific steroid hydroxylase. In Archives of biochemistry and biophysics, 340, 208-14. doi:. https://pubmed.ncbi.nlm.nih.gov/9143323/
4. Wang, Qiang, Titlow, William B, McClintock, Declan A, Stromberg, Arnold J, McClintock, Timothy S. . Activity-Dependent Gene Expression in the Mammalian Olfactory Epithelium. In Chemical senses, 42, 611-624. doi:10.1093/chemse/bjx028. https://pubmed.ncbi.nlm.nih.gov/28525560/
5. Gu, J, Zhang, Q Y, Genter, M B, Nebert, D W, Ding, X. . Purification and characterization of heterologously expressed mouse CYP2A5 and CYP2G1: role in metabolic activation of acetaminophen and 2,6-dichlorobenzonitrile in mouse olfactory mucosal microsomes. In The Journal of pharmacology and experimental therapeutics, 285, 1287-95. doi:. https://pubmed.ncbi.nlm.nih.gov/9618435/
6. Ling, Guoyu, Gu, Jun, Genter, Mary Beth, Zhuo, Xiaoliang, Ding, Xinxin. . Regulation of cytochrome P450 gene expression in the olfactory mucosa. In Chemico-biological interactions, 147, 247-58. doi:. https://pubmed.ncbi.nlm.nih.gov/15135081/
7. Yu, Tun-Tzu, McIntyre, Jeremy C, Bose, Soma C, Owen, Michael C, McClintock, Timothy S. . Differentially expressed transcripts from phenotypically identified olfactory sensory neurons. In The Journal of comparative neurology, 483, 251-62. doi:. https://pubmed.ncbi.nlm.nih.gov/15682396/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen