C57BL/6JCya-Cyp4a14em1flox/Cya
Common Name:
Cyp4a14-flox
Product ID:
S-CKO-01979
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cyp4a14-flox
Strain ID
CKOCMP-13119-Cyp4a14-B6J-VA
Gene Name
Product ID
S-CKO-01979
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp4a14em1flox/Cya mice (Catalog S-CKO-01979) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000030487
NCBI RefSeq
NM_007822
Target Region
Exon 2~3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Cyp4a14, a member of the cytochrome P450 (Cyp450) enzyme superfamily, possesses NADPH monooxygenase activity and catalyzes omega-hydroxylation of medium-chain fatty acids and arachidonic acid. It is associated with lipid metabolism and oxidative stress, and is a target marker gene of PPARα [1,3,4].
In various disease models, Cyp4a14 has been shown to play important roles. In a study on non-alcoholic steatohepatitis (NASH), Schisandra lignans alleviated hepatic damage by regulating lipid metabolism-related enzymes including Cyp4a14 [2]. In diabetic nephropathy, silencing Cyp4a14 by siRNA inhibited the proliferation and fibrosis of mesangial cells [4]. Cyp4a14-knockout (KO) mice had a higher number of goblet cells in the colon and were more resistant to dextran sulfate sodium (DSS)-induced colitis, suggesting Cyp4a14-increased oxidative stress exacerbates DSS-induced colitis [5]. Also, disruption of Cyp4a14 in mice led to hypertension, driven by excessive fluid reabsorption in the proximal tubule secondary to overexpression of NHE3 [6].
In conclusion, Cyp4a14 is crucial in lipid metabolism, oxidative stress and related pathological processes. Studies using Cyp4a14 KO mouse models have revealed its roles in diseases such as NASH, diabetic nephropathy, colitis and hypertension, providing potential targets for treatment of these diseases.
References:
1. Zhou, Fei, Yang, Linquan, Sun, Wenwen, Ma, Huijuan, Yang, Linlin. 2023. The PPARα/CYP4A14 bile acid pathway is associated with lipid metabolism disorders caused by low birth weight with high-fat diet. In Food & nutrition research, 67, . doi:10.29219/fnr.v67.8994. https://pubmed.ncbi.nlm.nih.gov/36794015/
2. Xue, Lijuan, Liu, Keanqi, Yan, Caixia, Wang, Guangji, Liang, Yan. 2023. Schisandra lignans ameliorate nonalcoholic steatohepatitis by regulating aberrant metabolism of phosphatidylethanolamines. In Acta pharmaceutica Sinica. B, 13, 3545-3560. doi:10.1016/j.apsb.2023.04.009. https://pubmed.ncbi.nlm.nih.gov/37655337/
3. Sobocanec, Sandra, Balog, Tihomir, Sarić, Ana, Kusić, Borka, Marotti, Tanja. . Cyp4a14 overexpression induced by hyperoxia in female CBA mice as a possible contributor of increased resistance to oxidative stress. In Free radical research, 44, 181-90. doi:10.3109/10715760903390820. https://pubmed.ncbi.nlm.nih.gov/19905990/
4. Wang, Suyu, Chen, Xin, Wang, Min, Yan, Qin, Lu, Weiping. 2017. SiRNA-Cyp4a14 and diabetic nephropathy: silencing of Cyp4a14 by siRNA inhibits proliferation and fibrosis of mesangial cells. In International journal of clinical and experimental pathology, 10, 11909-11917. doi:. https://pubmed.ncbi.nlm.nih.gov/31966555/
5. Xuan, Qingxia, Zhou, Yunfeng, Tan, Binbin, Chu, Fong-Fong, Gao, Qiang. 2018. Mice Deficient in Cyp4a14 Have An Increased Number of Goblet Cells and Attenuated Dextran Sulfate Sodium-Induced Colitis. In Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 50, 2272-2282. doi:10.1159/000495087. https://pubmed.ncbi.nlm.nih.gov/30423565/
6. Quigley, Raymond, Chakravarty, Sumana, Zhao, Xueying, Imig, John D, Capdevila, Jorge H. 2009. Increased renal proximal convoluted tubule transport contributes to hypertension in Cyp4a14 knockout mice. In Nephron. Physiology, 113, p23-8. doi:10.1159/000235774. https://pubmed.ncbi.nlm.nih.gov/19713718/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen