C57BL/6JCya-Cyp7b1em1flox/Cya
Common Name:
Cyp7b1-flox
Product ID:
S-CKO-01983
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cyp7b1-flox
Strain ID
CKOCMP-13123-Cyp7b1-B6J-VA
Gene Name
Product ID
S-CKO-01983
Gene Alias
D3Ertd552e; hct-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp7b1em1flox/Cya mice (Catalog S-CKO-01983) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035625
NCBI RefSeq
NM_007825
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Cyp7b1, a cytochrome P450 enzyme, hydroxylates carbons 6 and 7 of the B ring of oxysterols and steroids. This action reduces substrate biological activity and aids their conversion to excretable end products. It is involved in cholesterol metabolism, with its activity in the liver crucial for oxysterol inactivation and bile salt conversion [2].
In KO/CKO mouse models, Cyp7b1 has been found to play significant roles in multiple diseases. In osteoarthritis, adenoviral overexpression of Cyp7b1 in mouse joint tissues led to experimental osteoarthritis, while knockout or knockdown abrogated its pathogenesis, indicating its role in the CH25H-CYP7B1-RORα axis regulating osteoarthritis [1]. In the central nervous system autoimmune disease, CYP7B1 deficiency in mice significantly attenuated experimental autoimmune encephalomyelitis severity, reducing leukocyte infiltration, suppressing pathogenic CD4+ T cell proliferation, and decreasing myeloid cell activation [3]. In mesenchymal stem cells, BM-MSCs from CYP7B1 conditional knockout mice had impaired activation abilities, relating to delayed bone defect healing [4].
In conclusion, Cyp7B1 is essential for cholesterol metabolism, with its inactivation affecting multiple disease processes. Studies using KO/CKO mouse models have revealed its roles in osteoarthritis, central nervous system autoimmune diseases, and mesenchymal stem cell activation, providing potential therapeutic targets for these conditions.
References:
1. Choi, Wan-Su, Lee, Gyuseok, Song, Won-Hyun, Ryu, Je-Hwang, Chun, Jang-Soo. 2019. The CH25H-CYP7B1-RORα axis of cholesterol metabolism regulates osteoarthritis. In Nature, 566, 254-258. doi:10.1038/s41586-019-0920-1. https://pubmed.ncbi.nlm.nih.gov/30728500/
2. Stiles, Ashlee R, McDonald, Jeffrey G, Bauman, David R, Russell, David W. 2009. CYP7B1: one cytochrome P450, two human genetic diseases, and multiple physiological functions. In The Journal of biological chemistry, 284, 28485-9. doi:10.1074/jbc.R109.042168. https://pubmed.ncbi.nlm.nih.gov/19687010/
3. Song, Huanhuan, Lv, Aowei, Zhu, Zhibao, Wang, Ning, Fu, Ying. 2024. CYP7B1 deficiency impairs myeloid cell activation in autoimmune disease of the central nervous system. In PNAS nexus, 3, pgae334. doi:10.1093/pnasnexus/pgae334. https://pubmed.ncbi.nlm.nih.gov/39262855/
4. Zhang, Zhaoqiang, Su, Zepeng, Li, Zhikun, Shen, Huiyong, Xie, Zhongyu. 2024. CYP7B1-mediated 25-hydroxycholesterol degradation maintains quiescence-activation balance and improves therapeutic potential of mesenchymal stem cells. In Cell chemical biology, 31, 1277-1289.e7. doi:10.1016/j.chembiol.2024.01.009. https://pubmed.ncbi.nlm.nih.gov/38382532/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen