C57BL/6JCya-Dbpem1flox/Cya
Common Name:
Dbp-flox
Product ID:
S-CKO-02000
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dbp-flox
Strain ID
CKOCMP-13170-Dbp-B6J-VA
Gene Name
Product ID
S-CKO-02000
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dbpem1flox/Cya mice (Catalog S-CKO-02000) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080885
NCBI RefSeq
NM_016974
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Dbp can refer to multiple entities. One is the Vitamin D binding protein, which is a multifunctional protein present in blood. It is an actin-sequestering protein, binding to actin subdomains 1 and 3, blocking the fast-growing side of actin monomers, and effectively interfering with actin-filament formation [5]. Another refers to Dibutyl phthalate, an endocrine-disrupting chemical widely used in industry [2].
In the context of Dibutyl phthalate, studies show it has detrimental effects. In rats, co-exposure to DBP and BaP causes more severe kidney injury than single-exposure, through promoting pyroptosis of renal tubular epithelial cells regulated by TLR4/NF-κB signaling [1]. In mouse Leydig cells, DBP induces ferroptosis via upregulating Sp2, which promotes Vdac2 transcription, while melatonin alleviates this by inhibiting Sp2/VDAC2 signals [2]. Pre-conception exposure to DBP in rodents impairs spermatogenesis by activating NF-κB/COX-2/RANKL signaling in Sertoli cells [3]. Also, in zebrafish, DBP exposure during embryonic development causes craniofacial defects [4].
In conclusion, the effects of Dbp (Dibutyl phthalate) are diverse and involve multiple biological processes and disease-related conditions. These findings from in vivo studies on animals like rats, mice, and zebrafish help understand its role in causing damage to organs such as the kidney and testis, as well as affecting embryonic development.
References:
1. Chen, Jing, Song, Yawen, Liu, Yining, You, Mingdan, Yang, Guanghong. 2022. DBP and BaP co-exposure induces kidney injury via promoting pyroptosis of renal tubular epithelial cells in rats. In Chemosphere, 314, 137714. doi:10.1016/j.chemosphere.2022.137714. https://pubmed.ncbi.nlm.nih.gov/36592837/
2. Yang, Si, Chen, Meiwei, Meng, Jiahui, Wang, Jinglei, Chen, Jiaxiang. 2024. Melatonin alleviates di-butyl phthalate (DBP)-induced ferroptosis of mouse leydig cells via inhibiting Sp2/VDAC2 signals. In Environmental research, 247, 118221. doi:10.1016/j.envres.2024.118221. https://pubmed.ncbi.nlm.nih.gov/38246300/
3. Xie, Zhiwen, Liu, Shiwei, Hua, Shan, Shi, Fei, Jiang, Juntao. 2022. Preconception exposure to dibutyl phthalate (DBP) impairs spermatogenesis by activating NF-κB/COX-2/RANKL signaling in Sertoli cells. In Toxicology, 474, 153213. doi:10.1016/j.tox.2022.153213. https://pubmed.ncbi.nlm.nih.gov/35605887/
4. Jergensen, Tanner, Cusmano, Danielle, Roy, Nicole M. 2019. Di-butyl phthalate (DBP) induces craniofacial defects during embryonic development in zebrafish. In Ecotoxicology (London, England), 28, 995-1002. doi:10.1007/s10646-019-02100-7. https://pubmed.ncbi.nlm.nih.gov/31463621/
5. Verboven, Christel, Bogaerts, Ilse, Waelkens, Etienne, Bouillon, Roger, De Ranter, Camiel. 2003. Actin-DBP: the perfect structural fit? In Acta crystallographica. Section D, Biological crystallography, 59, 263-73. doi:. https://pubmed.ncbi.nlm.nih.gov/12554937/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen