C57BL/6JCya-Dnmt1em1flox/Cya
Common Name:
Dnmt1-flox
Product ID:
S-CKO-02072
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dnmt1-flox
Strain ID
CKOCMP-13433-Dnmt1-B6J-VA
Gene Name
Product ID
S-CKO-02072
Gene Alias
Cxxc9; Dnmt; Dnmt1o; MCMT; MTase; Met-1; Met1; MommeD2; m.MmuI
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dnmt1em1flox/Cya mice (Catalog S-CKO-02072) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000216540
NCBI RefSeq
NM_001199433
Target Region
Exon 5
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Dnmt1, DNA methyltransferase 1, is the predominant member of the DNMT family and the most abundant DNMT in various cell types. It functions as a maintenance DNMT, playing a crucial role in regulating DNA methylation, which is involved in multiple biological processes such as chromatin organization, DNA repair, cell cycle regulation, and apoptosis [2,3].
In triple-negative breast cancer (TNBC), Dnmt1 is overexpressed and associated with poor survival. It represses estrogen receptor (ER) expression, promotes epithelial-mesenchymal transition (EMT) for metastasis, induces cellular autophagy, and promotes the growth of cancer stem cells by hypermethylating relevant promoter regions [1]. In human erythropoiesis, Dnmt1 deficiency leads to cell cycle arrest of erythroid progenitors due to decreased DNA methylation of the p21 promoter and increased apoptosis during the terminal stage by inducing endoplasmic reticulum (ER) stress [4]. In esophageal cancer, Dnmt1 negatively correlates with FBXO32 expression, and its enrichment in the FBXO32 promoter region leads to increased DNA methylation and reduced transcription, promoting cancer cell growth [5].
In conclusion, Dnmt1 is essential for maintaining normal DNA methylation patterns and is involved in numerous biological processes. Studies, including those using potential gene knockout models inferred from the functional impacts observed, have revealed its significant roles in cancer development such as in TNBC, esophageal cancer, and in processes like erythropoiesis. Understanding Dnmt1 provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Wong, Kah Keng. 2020. DNMT1: A key drug target in triple-negative breast cancer. In Seminars in cancer biology, 72, 198-213. doi:10.1016/j.semcancer.2020.05.010. https://pubmed.ncbi.nlm.nih.gov/32461152/
2. Yan, Lan, Geng, Qi, Cao, Zhiwen, Zhao, Ning, Lu, Cheng. 2023. Insights into DNMT1 and programmed cell death in diseases. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 168, 115753. doi:10.1016/j.biopha.2023.115753. https://pubmed.ncbi.nlm.nih.gov/37871559/
3. Svedružić, Željko M. . Dnmt1 structure and function. In Progress in molecular biology and translational science, 101, 221-54. doi:10.1016/B978-0-12-387685-0.00006-8. https://pubmed.ncbi.nlm.nih.gov/21507353/
4. Yang, Qianqian, Chen, Lixiang, Zhang, Hengchao, An, Xiuli, Wang, Ting. 2024. DNMT1 regulates human erythropoiesis by modulating cell cycle and endoplasmic reticulum stress in a stage-specific manner. In Cell death and differentiation, 31, 999-1012. doi:10.1038/s41418-024-01305-6. https://pubmed.ncbi.nlm.nih.gov/38719927/
5. Song, Xian-Qiang, Chen, Bin-Bin, Jin, Yong-Mei, Wang, Chang-Yong. 2024. DNMT1-mediated epigenetic suppression of FBXO32 expression promoting cyclin dependent kinase 9 (CDK9) survival and esophageal cancer cell growth. In Cell cycle (Georgetown, Tex.), 23, 262-278. doi:10.1080/15384101.2024.2309022. https://pubmed.ncbi.nlm.nih.gov/38597826/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen