C57BL/6JCya-Dnmt3aem1flox/Cya
Common Name:
Dnmt3a-flox
Product ID:
S-CKO-02074
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Dnmt3a-flox
Strain ID
CKOCMP-13435-Dnmt3a-B6J-VA
Gene Name
Product ID
S-CKO-02074
Gene Alias
MmuIIIA
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dnmt3aem1flox/Cya mice (Catalog S-CKO-02074) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020991
NCBI RefSeq
NM_007872
Target Region
Exon 9~12
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Dnmt3a, a de novo DNA methyltransferase, is crucial for DNA methylation, an epigenetic process involved in development, aging, and cancer [1,2,4]. DNA methylation by Dnmt3a at cytosines is essential for genome regulation [2]. It plays a significant role in controlling hematopoietic stem cell fate decisions [1].
In mouse chimeras, inactivation of Dnmt3a (Dnmt3a -/-) led to substantial expansion of Dnmt3a -/- hematopoietic stem cells (HSCs) during chronic mycobacterial infection, indicating that IFNγ signaling induced during chronic infection can drive Dnmt3a -loss -of -function clonal hematopoiesis [3]. In another study, DNMT3AR882H, a common Dnmt3a mutation in angioimmunoblastic T -cell lymphoma (AITL), accelerated the development of Tet2 -/-; RHOAG17V AITL in mice, with single -cell transcriptome analyses revealing enhanced interactions between Tfh and B cells [5]. Also, in a stroke mouse model, increased Dnmt3a protein levels were observed in the brain penumbra, and inhibition of Dnmt3a amplified neutrophil-related inflammation, increased infarct volume, and worsened neurobehavioral function [6].
In conclusion, Dnmt3a is essential for DNA methylation and has a significant impact on processes like hematopoietic stem cell fate determination, clonal hematopoiesis, and responses in diseases such as AITL and acute ischemic stroke. Mouse models, especially those with Dnmt3a inactivation or mutation, have been instrumental in revealing these roles, contributing to our understanding of the underlying mechanisms in these disease areas.
References：
1. Brunetti, Lorenzo, Gundry, Michael C, Goodell, Margaret A. 2017. DNMT3A in Leukemia. In Cold Spring Harbor perspectives in medicine, 7, . doi:10.1101/cshperspect.a030320. https://pubmed.ncbi.nlm.nih.gov/28003281/
2. Zhang, Zhi-Min, Lu, Rui, Wang, Pengcheng, Wang, Gang Greg, Song, Jikui. 2018. Structural basis for DNMT3A-mediated de novo DNA methylation. In Nature, 554, 387-391. doi:10.1038/nature25477. https://pubmed.ncbi.nlm.nih.gov/29414941/
3. Hormaechea-Agulla, Daniel, Matatall, Katie A, Le, Duy T, Kimmel, Marek, King, Katherine Y. 2021. Chronic infection drives Dnmt3a-loss-of-function clonal hematopoiesis via IFNγ signaling. In Cell stem cell, 28, 1428-1442.e6. doi:10.1016/j.stem.2021.03.002. https://pubmed.ncbi.nlm.nih.gov/33743191/
4. Okano, M, Bell, D W, Haber, D A, Li, E. . DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development. In Cell, 99, 247-57. doi:. https://pubmed.ncbi.nlm.nih.gov/10555141/
5. Zheng, Jianan, Wang, Zhongwang, Pan, Xiangyu, Niu, Ting, Liu, Yu. 2023. DNMT3AR882H accelerates angioimmunoblastic T-cell lymphoma in mice. In Oncogene, 42, 1940-1950. doi:10.1038/s41388-023-02699-2. https://pubmed.ncbi.nlm.nih.gov/37127775/
6. Lyu, Tian-Jie, Qiu, Xin, Wang, Yubo, Wang, Yongjun, Li, Zixiao. 2024. DNMT3A dysfunction promotes neuroinflammation and exacerbates acute ischemic stroke. In MedComm, 5, e652. doi:10.1002/mco2.652. https://pubmed.ncbi.nlm.nih.gov/39006763/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen