C57BL/6JCya-Drd4em1flox/Cya
Common Name:
Drd4-flox
Product ID:
S-CKO-02093
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Drd4-flox
Strain ID
CKOCMP-13491-Drd4-B6J-VA
Gene Name
Product ID
S-CKO-02093
Gene Alias
D4R; Drd-4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Drd4em1flox/Cya mice (Catalog S-CKO-02093) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000026569
NCBI RefSeq
NM_007878
Target Region
Exon 2~4
Size of Effective Region
~3.4 kb
Detailed Document
Overview of Gene Research
Drd4, encoding the dopamine D4 receptor, is involved in dopamine-related signaling pathways. Dopamine signaling is crucial for various physiological and behavioral processes, including regulation of mood, reward-seeking behavior, and motor control [4].
In acute kidney injury (AKI), Drd4 was found to be decreased in mouse models of ischemia/reperfusion injury or cisplatin treatment. Activation of Drd4 attenuated nephrotoxicity by suppressing oxidative stress and enhancing mitochondrial bioenergetics through down-regulating ROS generation and NOX4 expression. Mechanistically, it reduced the expression of ISG15, suppressing NOX4 ISGylation and enhancing its ubiquitination for degradation [1].
In liver cancer, Drd4 expression is elevated in liver cancer stem cells (LCSCs) and promotes chemo-resistance and CSC-like phenotypes via activating the PI3K/Akt/GSK-3β pathway to stabilize β-catenin and promote its nuclear entry [2].
In myocardial ischemia/reperfusion injury, Drd4 agonist improved cardiac function, reduced heart damage, and apoptosis in cardiomyocytes by promoting GLUT4 translocation depending on the PI3K/AKT pathway [3].
In conclusion, Drd4 plays essential roles in multiple physiological and disease-related processes. Its functions in protecting against AKI, promoting liver cancer stem cell phenotypes, and mitigating myocardial ischemia/reperfusion injury have been revealed through in vivo and in vitro studies, mainly in mouse models. Understanding Drd4 may provide new therapeutic targets for these diseases.
References：
1. Gao, Yue, Lu, Xun, Zhang, Guangyuan, Chen, Shuqiu, Zhang, Lei. 2024. DRD4 alleviates acute kidney injury by suppressing ISG15/NOX4 axis-associated oxidative stress. In Redox biology, 70, 103078. doi:10.1016/j.redox.2024.103078. https://pubmed.ncbi.nlm.nih.gov/38354631/
2. Yang, Zhengyan, Zhang, Pai, Zhao, Yiwei, Hu, Yanzhong, Cui, Daxiang. 2024. DRD4 promotes chemo-resistance and cancer stem cell-like phenotypes by mediating the activation of the Akt/β-catenin signaling axis in liver cancer. In British journal of cancer, 131, 1212-1223. doi:10.1038/s41416-024-02811-7. https://pubmed.ncbi.nlm.nih.gov/39174739/
3. Liu, Xue-Song, Zeng, Jing, Yang, Yu-Xue, Zeng, Chun-Yu, Wang, Da-Xin. 2021. DRD4 Mitigates Myocardial Ischemia/Reperfusion Injury in Association With PI3K/AKT Mediated Glucose Metabolism. In Frontiers in pharmacology, 11, 619426. doi:10.3389/fphar.2020.619426. https://pubmed.ncbi.nlm.nih.gov/33584304/
4. Koob, Andrew O, Ballantyne, Scott, Levesque, Aime A, Qureshi, Amber A, Congdon, Sean. 2021. DRD4 Allele Frequency as a Lab Exercise in Neuroscience and Genetics Courses. In Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience, 20, A1-A10. doi:. https://pubmed.ncbi.nlm.nih.gov/35540948/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen