C57BL/6JCya-Ebf1em1flox/Cya
Common Name:
Ebf1-flox
Product ID:
S-CKO-02125
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ebf1-flox
Strain ID
CKOCMP-13591-Ebf1-B6J-VA
Gene Name
Product ID
S-CKO-02125
Gene Alias
Ebf; O/E-1; OE-1; Olf-1; Olf1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ebf1em1flox/Cya mice (Catalog S-CKO-02125) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101326
NCBI RefSeq
NM_001290709
Target Region
Exon 2~4
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
Ebf1, also known as early B cell factor 1, is a crucial transcription factor. It plays a vital role in early B cell lymphopoiesis, acting as a key regulator of B cell specification [1,2,4,5]. Ebf1 is involved in processes like establishing and stabilizing chromatin accessibility in lymphoid progenitors and pro-B cells, which is essential for lineage-specific gene expression changes during B cell differentiation [3,5]. It also participates in the regulation of genes related to V(D)J recombination, a process fundamental to the development of a diverse lymphocyte antigen receptor repertoire in adaptive immunity [1,2].
In gene knockout (KO) mouse models, Ebf1 deficiency leads to significant impacts on B cell development. Acute protein degradation in mice revealed that Ebf1, along with other transcription factors, controls early B cell lymphopoiesis. Ebf1 predominantly functions as a transcriptional activator, inducing open chromatin at its target genes such as Igll1 and Vpreb1 in pro-B cells [1]. In pro-B cells, replacing Ebf1 with a degradable form showed that continuous Ebf1 activity is required for maintaining the transcriptional and epigenetic state of these cells [3]. Additionally, in hematopoietic cells, Ebf1 deletion enhanced myelopoiesis and reduced HSC repopulation capacity, suggesting its role in regulating myeloid/lymphoid fate bias in multipotent progenitors [6].
In conclusion, Ebf1 is essential for early B cell development, influencing chromatin accessibility, gene expression, and the balance between myeloid and lymphoid lineages. The use of KO mouse models has been instrumental in uncovering these functions, providing insights into the pathogenesis of B-cell-related hematologic neoplasms and potentially other diseases associated with abnormal B cell development [1,2,6].
References:
1. Fedl, Anna S, Tagoh, Hiromi, Gruenbacher, Sarah, Busslinger, Meinrad, Schwickert, Tanja A. 2024. Transcriptional function of E2A, Ebf1, Pax5, Ikaros and Aiolos analyzed by in vivo acute protein degradation in early B cell development. In Nature immunology, 25, 1663-1677. doi:10.1038/s41590-024-01933-7. https://pubmed.ncbi.nlm.nih.gov/39179932/
2. Li, Li, Zhang, Daiquan, Cao, Xinmei. 2024. EBF1, PAX5, and MYC: regulation on B cell development and association with hematologic neoplasms. In Frontiers in immunology, 15, 1320689. doi:10.3389/fimmu.2024.1320689. https://pubmed.ncbi.nlm.nih.gov/38318177/
3. Zolotarev, Nikolay, Bayer, Marc, Grosschedl, Rudolf. 2022. EBF1 is continuously required for stabilizing local chromatin accessibility in pro-B cells. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2210595119. doi:10.1073/pnas.2210595119. https://pubmed.ncbi.nlm.nih.gov/36409886/
4. Bayer, Marc, Boller, Sören, Ramamoothy, Senthilkumar, Boehm, Thomas, Grosschedl, Rudolf. 2022. Tnpo3 enables EBF1 function in conditions of antagonistic Notch signaling. In Genes & development, 36, 901-915. doi:10.1101/gad.349696.122. https://pubmed.ncbi.nlm.nih.gov/36167471/
5. Boller, Sören, Li, Rui, Grosschedl, Rudolf. 2018. Defining B Cell Chromatin: Lessons from EBF1. In Trends in genetics : TIG, 34, 257-269. doi:10.1016/j.tig.2017.12.014. https://pubmed.ncbi.nlm.nih.gov/29336845/
6. Lenaerts, Aurelie, Kucinski, Iwo, Deboutte, Ward, Göttgens, Berthold, Grosschedl, Rudolf. 2022. EBF1 primes B-lymphoid enhancers and limits the myeloid bias in murine multipotent progenitors. In The Journal of experimental medicine, 219, . doi:10.1084/jem.20212437. https://pubmed.ncbi.nlm.nih.gov/36048017/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen