C57BL/6JCya-Eef2em1flox/Cya
Common Name:
Eef2-flox
Product ID:
S-CKO-02149
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Eef2-flox
Strain ID
CKOCMP-13629-Eef2-B6J-VA
Gene Name
Product ID
S-CKO-02149
Gene Alias
Ef-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Eef2em1flox/Cya mice (Catalog S-CKO-02149) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000047864
NCBI RefSeq
NM_007907
Target Region
Exon 2~3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Eef2, or eukaryotic elongation factor 2, is a key ribosomal protein that catalyzes the elongation phase of protein translation, a fundamental process in all cells. It is ubiquitously expressed and is crucial for neuronal function among other biological processes. Post-translational modifications (PTMs) of eEF2, such as phosphorylation and methylation, can significantly influence mRNA translation dynamics [1,2,5,6].
In lung adenocarcinoma (LUAD), FAM86A-mediated methylation of eEF2 at K525 (eEF2K525me3) promotes protein synthesis and disease pathogenesis. Depletion of FAM86A in LUAD cells leads to 80S monosome accumulation and translation inhibition, and knockdown of FAM86A attenuates LUAD cell proliferation and xenograft growth in vivo [1].
In the context of depression, LPS-induced depression-like behaviors in mice are accompanied by reduced eEF2 activity, while fluoxetine treatment can reverse this and exhibit antidepressant effects, suggesting an involvement of eEF2 in the antidepressant mechanisms related to HDAC1-eEF2-associated neuroinflammation and synaptogenesis [2].
In megakaryocyte (MK) maturation and platelet production, lactate dehydrogenase A (LdhA) interacts with eEF2 in the cytoplasm, and inhibitors of LDHA that release eEF2 can enhance MK maturation and platelet production in vitro and in vivo [3].
In chronic infections by Pseudomonas aeruginosa, exotoxin A (EXOA)-driven ribosylation and inactivation of EEF2 promote ribotoxic stress and NLRP1 inflammasome activation in human airway and corneal epithelial cells [4].
In conclusion, Eef2 is essential for protein translation elongation and has far-reaching impacts on various biological processes and disease conditions. The use of gene knockout and other loss-of-function models, as seen in LUAD mouse models, has provided valuable insights into the role of Eef2 in cancer development. These findings highlight the significance of Eef2 in understanding disease mechanisms and potentially developing new therapeutic strategies.
References:
1. Francis, Joel William, Hausmann, Simone, Ikram, Sabeen, Mazur, Pawel Karol, Gozani, Or. 2024. FAM86A methylation of eEF2 links mRNA translation elongation to tumorigenesis. In Molecular cell, 84, 1753-1763.e7. doi:10.1016/j.molcel.2024.02.037. https://pubmed.ncbi.nlm.nih.gov/38508183/
2. Li, Weifen, Ali, Tahir, Zheng, Chengyou, Yu, Zhi-Jian, Li, Shupeng. 2021. Fluoxetine regulates eEF2 activity (phosphorylation) via HDAC1 inhibitory mechanism in an LPS-induced mouse model of depression. In Journal of neuroinflammation, 18, 38. doi:10.1186/s12974-021-02091-5. https://pubmed.ncbi.nlm.nih.gov/33526073/
3. Chen, Qidi, Xin, Min, Wang, Lingjun, Liu, Junling, Fan, Xuemei. . Inhibition of LDHA to induce eEF2 release enhances thrombocytopoiesis. In Blood, 139, 2958-2971. doi:10.1182/blood.2022015620. https://pubmed.ncbi.nlm.nih.gov/35176139/
4. Pinilla, Miriam, Mazars, Raoul, Vergé, Romain, Planès, Rémi, Meunier, Etienne. 2023. EEF2-inactivating toxins engage the NLRP1 inflammasome and promote epithelial barrier disruption. In The Journal of experimental medicine, 220, . doi:10.1084/jem.20230104. https://pubmed.ncbi.nlm.nih.gov/37642996/
5. Suzuki, Kanzo, Monteggia, Lisa M. 2020. The role of eEF2 kinase in the rapid antidepressant actions of ketamine. In Advances in pharmacology (San Diego, Calif.), 89, 79-99. doi:10.1016/bs.apha.2020.04.005. https://pubmed.ncbi.nlm.nih.gov/32616215/
6. Henis, Melad, Rücker, Tabitha, Scharrenberg, Robin, Duncan, Kent E, de Anda, Froylan Calderón. 2024. The autism susceptibility kinase, TAOK2, phosphorylates eEF2 and modulates translation. In Science advances, 10, eadf7001. doi:10.1126/sciadv.adf7001. https://pubmed.ncbi.nlm.nih.gov/38608030/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen