C57BL/6JCya-Efnb2em1flox/Cya
Common Name:
Efnb2-flox
Product ID:
S-CKO-02157
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Efnb2-flox
Strain ID
CKOCMP-13642-Efnb2-B6J-VA
Gene Name
Product ID
S-CKO-02157
Gene Alias
ELF-2; Epl5; Eplg5; Htk-L; LERK-5; Lerk5; NLERK-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Efnb2em1flox/Cya mice (Catalog S-CKO-02157) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001319
NCBI RefSeq
NM_010111
Target Region
Exon 2
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Efnb2, encoding the transmembrane protein ephrin-B2, is involved in multiple crucial biological functions. It plays a role in cell-to-cell communication pathways, such as the EFNB2-EPHB4 axis which is part of a direct interaction between osteoblasts and osteoclasts, regulating their differentiation and survival, thus contributing to bone homeostasis [1]. Ephrin-B2 also has a role in the assembly of connexin 30 (Cx30) gap junction plaques in the cochlea, and its haploinsufficiency can lead to early disassembly and endocytosis of these plaques, potentially contributing to sensorineural hearing loss [2].
In disease-related research, Efnb2 has been found to be involved in various conditions. In head and neck cancer, it is a key regulator of cetuximab resistance, and targeting it and associated protein-protein interaction circuits might improve treatment response [3]. In colorectal cancer liver metastases, the EFNB2/EPHB4 axis promotes post-metastatic growth via LDLR-mediated cholesterol uptake [4]. In pancreatic ductal adenocarcinoma, EFNB2 promotes cell proliferation, migration, and invasion through the p53/p21 pathway and epithelial-mesenchymal transition (EMT), making it a potential diagnostic and treatment target [6]. In laryngeal squamous cell carcinoma, the mTORC1-ITGA5-EFNB2 signaling pathway is associated with malignant progression and poor prognosis [7]. In intrahepatic cholangiocarcinoma, the Dll4-Notch4-Efnb2 signaling is involved in the differentiation of periportal liver sinusoidal endothelial cells into tip endothelial cells to promote cancer development [8]. Also, EFNB2 potentially protects against intervertebral disc degeneration by inhibiting nucleus pulposus cell apoptosis [5].
In conclusion, Efnb2 is essential for multiple biological processes including cell communication and tissue homeostasis. Its dysregulation is associated with various diseases such as cancers and hearing loss. Studies on Efnb2, especially through gene-knockout or conditional-knockout mouse models in relevant research, have provided valuable insights into its functions in normal and diseased states, highlighting its potential as a therapeutic target for these diseases.
References：
1. Kim, Jung-Min, Lin, Chujiao, Stavre, Zheni, Greenblatt, Matthew B, Shim, Jae-Hyuck. 2020. Osteoblast-Osteoclast Communication and Bone Homeostasis. In Cells, 9, . doi:10.3390/cells9092073. https://pubmed.ncbi.nlm.nih.gov/32927921/
2. Defourny, Jean, Audouard, Christophe, Davy, Alice, Thiry, Marc. 2021. Efnb2 haploinsufficiency induces early gap junction plaque disassembly and endocytosis in the cochlea. In Brain research bulletin, 174, 153-160. doi:10.1016/j.brainresbull.2021.06.008. https://pubmed.ncbi.nlm.nih.gov/34139316/
3. Chaudhary, Raushan Kumar, Patil, Prakash, Mateti, Uday Venkat, Alagundagi, Dhananjay B, Shetty, Vijith. 2023. Theranostic Potential of EFNB2 for Cetuximab Resistance in Head and Neck Cancer. In Indian journal of otolaryngology and head and neck surgery : official publication of the Association of Otolaryngologists of India, 75, 1923-1936. doi:10.1007/s12070-023-03739-9. https://pubmed.ncbi.nlm.nih.gov/37636764/
4. Xu, Chunjie, Gu, Lei, Kuerbanjiang, Manzila, Zhang, Zhigang, Xu, Qing. 2022. Adaptive activation of EFNB2/EPHB4 axis promotes post-metastatic growth of colorectal cancer liver metastases by LDLR-mediated cholesterol uptake. In Oncogene, 42, 99-112. doi:10.1038/s41388-022-02519-z. https://pubmed.ncbi.nlm.nih.gov/36376513/
5. Zhang, Qianshi, Li, Jing, Liu, Fubing, Zou, Jianfei, Wang, Xiaobin. 2024. Ephrin B2 (EFNB2) potentially protects against intervertebral disc degeneration through inhibiting nucleus pulposus cell apoptosis. In Archives of biochemistry and biophysics, 756, 109990. doi:10.1016/j.abb.2024.109990. https://pubmed.ncbi.nlm.nih.gov/38636690/
6. Zhu, Feng, Dai, Shang-Nan, Xu, Da-Lai, Wu, Jun-Li, Miao, Yi. 2020. EFNB2 facilitates cell proliferation, migration, and invasion in pancreatic ductal adenocarcinoma via the p53/p21 pathway and EMT. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 125, 109972. doi:10.1016/j.biopha.2020.109972. https://pubmed.ncbi.nlm.nih.gov/32036221/
7. Li, Dapeng, Sun, Anjiang, Zhang, Liang, Zha, Xiaojun, Liu, Yehai. 2022. Elevated ITGA5 facilitates hyperactivated mTORC1-mediated progression of laryngeal squamous cell carcinoma via upregulation of EFNB2. In Theranostics, 12, 7431-7449. doi:10.7150/thno.76232. https://pubmed.ncbi.nlm.nih.gov/36438491/
8. Wang, Tingjie, Xu, Chuanrui, Zhang, Zhijing, Li, Lei, Ye, Kai. 2022. Cellular heterogeneity and transcriptomic profiles during intrahepatic cholangiocarcinoma initiation and progression. In Hepatology (Baltimore, Md.), 76, 1302-1317. doi:10.1002/hep.32483. https://pubmed.ncbi.nlm.nih.gov/35340039/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen