C57BL/6NCya-Eif4a1em1flox/Cya
Common Name:
Eif4a1-flox
Product ID:
S-CKO-02176
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Eif4a1-flox
Strain ID
CKOCMP-13681-Eif4a1-B6N-VA
Gene Name
Product ID
S-CKO-02176
Gene Alias
BM-010; Ddx2a; Eif4
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Eif4a1em1flox/Cya mice (Catalog S-CKO-02176) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000163666
NCBI RefSeq
NM_144958
Target Region
Exon 2~4
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Eif4a1, a DEAD-box RNA-binding protein, is an essential eukaryotic translation initiation factor. It plays a crucial role in translation initiation by unwinding the secondary structure of mRNA upstream of the start codon, enabling ribosomal recruitment for downstream gene translation [2,4]. It is involved in pathways related to cell proliferation, such as the mTORC1 pathway [2,5,6].
Using mouse models to delete Eif4a1 in B cells, it was found that Eif4a1 is essential for B cell development and the germinal centre response. After B cell activation in vitro, Eif4a1 facilitates an increased rate of protein synthesis, MYC expression, and expression of cell cycle regulators [3]. In triple-negative breast cancer, IGF2BP2 recruits Eif4a1 to promote the translation output of CDK6, driving cell cycle progression [1].
In conclusion, Eif4a1 is vital for translation initiation and significantly impacts cell-related biological processes. Gene-knockout mouse models have revealed its role in B-cell-related processes and in triple-negative breast cancer, providing insights into its function in normal and disease-related conditions.
References：
1. Xia, Tian, Dai, Xin-Yuan, Sang, Ming-Yi, Wei, Ji-Fu, Ding, Qiang. 2023. IGF2BP2 Drives Cell Cycle Progression in Triple-Negative Breast Cancer by Recruiting EIF4A1 to Promote the m6A-Modified CDK6 Translation Initiation Process. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2305142. doi:10.1002/advs.202305142. https://pubmed.ncbi.nlm.nih.gov/37983610/
2. Shichino, Yuichi, Yamaguchi, Tomokazu, Kashiwagi, Kazuhiro, Kuba, Keiji, Iwasaki, Shintaro. 2024. eIF4A1 enhances LARP1-mediated translational repression during mTORC1 inhibition. In Nature structural & molecular biology, 31, 1557-1566. doi:10.1038/s41594-024-01321-7. https://pubmed.ncbi.nlm.nih.gov/38773334/
3. Screen, Michael, Matheson, Louise S, Howden, Andrew Jm, Sansom, Owen, Turner, Martin. 2023. RNA helicase EIF4A1-mediated translation is essential for the GC response. In Life science alliance, 7, . doi:10.26508/lsa.202302301. https://pubmed.ncbi.nlm.nih.gov/38011999/
4. Kayastha, Forum, Herrington, Noah B, Kapadia, Bandish, Kellogg, Glen E, Gartenhaus, Ronald B. 2022. Novel eIF4A1 inhibitors with anti-tumor activity in lymphoma. In Molecular medicine (Cambridge, Mass.), 28, 101. doi:10.1186/s10020-022-00534-0. https://pubmed.ncbi.nlm.nih.gov/36058921/
5. Jiao, Dongyue, Sun, Huiru, Zhao, Xiaying, Wang, Chenji, Gao, Kun. 2024. mTORC1/S6K1 signaling promotes sustained oncogenic translation through modulating CRL3IBTK-mediated ubiquitination of eIF4A1 in cancer cells. In eLife, 12, . doi:10.7554/eLife.92236. https://pubmed.ncbi.nlm.nih.gov/38738857/
6. Han, Luyang, Wu, Yuting, Liu, Fangming, Zhang, Hongbing. 2022. eIF4A1 Inhibitor Suppresses Hyperactive mTOR-Associated Tumors by Inducing Necroptosis and G2/M Arrest. In International journal of molecular sciences, 23, . doi:10.3390/ijms23136932. https://pubmed.ncbi.nlm.nih.gov/35805935/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen