C57BL/6JCya-Ephb4em1flox/Cya
Common Name:
Ephb4-flox
Product ID:
S-CKO-02229
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ephb4-flox
Strain ID
CKOCMP-13846-Ephb4-B6J-VA
Gene Name
Product ID
S-CKO-02229
Gene Alias
Htk; MDK2; Myk1; Tyro11; b2b2412Clo
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ephb4em1flox/Cya mice (Catalog S-CKO-02229) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111055
NCBI RefSeq
NM_001159571
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
EphB4, a member of the Eph receptor family of receptor tyrosine kinases, plays crucial roles in multiple biological processes. It is involved in cell adhesion and migration, and its signaling pathways, such as EphrinB2-EphB4, are critical for cardiovascular formation, neuronal guidance during embryogenesis [1,2,3,6,7]. This signaling also intersects with the RAS-MAPK pathway, which is important in various cellular functions [4,5].
In animal models, LV-specific inducible EphB4-deficient mice and EphB4 knockin mice (EphB4 2YP) were used to study its role in lymphatic vessel (LV) valve development. Loss of EphB4 in LV blocked LV valve specification, continued valve development, and valve maintenance. In these models, defects in LV valve development were reversed by inhibiting the Ras-MAPK signaling pathway, indicating that EphB4-RASA1 interaction is necessary to limit Ras-MAPK activation in lymphatic endothelial cells [5].
In conclusion, EphB4 is essential for cardiovascular and neuronal development, as well as LV valve development. Mouse models, such as the EphB4-deficient and EphB4 knockin mice, have provided valuable insights into its role in these processes and the associated signaling pathways, contributing to our understanding of related physiological and pathological conditions.
References：
1. Piffko, Andras, Uhl, Christian, Vajkoczy, Peter, Czabanka, Marcus, Broggini, Thomas. 2022. EphrinB2-EphB4 Signaling in Neurooncological Disease. In International journal of molecular sciences, 23, . doi:10.3390/ijms23031679. https://pubmed.ncbi.nlm.nih.gov/35163601/
2. Zeng, Xue, Hunt, Ava, Jin, Sheng Chih, Gaillard, Jonathan, Kahle, Kristopher T. 2019. EphrinB2-EphB4-RASA1 Signaling in Human Cerebrovascular Development and Disease. In Trends in molecular medicine, 25, 265-286. doi:10.1016/j.molmed.2019.01.009. https://pubmed.ncbi.nlm.nih.gov/30819650/
3. Chen, Yinnan, Zhang, Hongmei, Zhang, Yanmin. 2017. Targeting receptor tyrosine kinase EphB4 in cancer therapy. In Seminars in cancer biology, 56, 37-46. doi:10.1016/j.semcancer.2017.10.002. https://pubmed.ncbi.nlm.nih.gov/28993206/
4. Amyere, Mustapha, Revencu, Nicole, Helaers, Raphaël, Boon, Laurence M, Vikkula, Miikka. 2017. Germline Loss-of-Function Mutations in EPHB4 Cause a Second Form of Capillary Malformation-Arteriovenous Malformation (CM-AVM2) Deregulating RAS-MAPK Signaling. In Circulation, 136, 1037-1048. doi:10.1161/CIRCULATIONAHA.116.026886. https://pubmed.ncbi.nlm.nih.gov/28687708/
5. Chen, Di, Tang, Yipei, Lapinski, Philip E, Davis, Michael J, King, Philip D. 2024. EPHB4-RASA1 Inhibition of PIEZO1 Ras Activation Drives Lymphatic Valvulogenesis. In Circulation research, 135, 1048-1066. doi:10.1161/CIRCRESAHA.124.325383. https://pubmed.ncbi.nlm.nih.gov/39421925/
6. Ullah, Asmat, Razzaq, Anam, Zhou, Chuanzan, Elbehairi, Serag Eldin I, Iqbal, Haroon. . Biological Significance of EphB4 Expression in Cancer. In Current protein & peptide science, 25, 244-255. doi:10.2174/0113892037269589231017055642. https://pubmed.ncbi.nlm.nih.gov/37909437/
7. Du, Enming, Li, Xue, He, Siyu, Li, Xiaohua, He, Shikun. 2020. The critical role of the interplays of EphrinB2/EphB4 and VEGF in the induction of angiogenesis. In Molecular biology reports, 47, 4681-4690. doi:10.1007/s11033-020-05470-y. https://pubmed.ncbi.nlm.nih.gov/32488576/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen