C57BL/6JCya-Ercc1em1flox/Cya
Common Name:
Ercc1-flox
Product ID:
S-CKO-02249
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ercc1-flox
Strain ID
CKOCMP-13870-Ercc1-B6J-VA
Gene Name
Product ID
S-CKO-02249
Gene Alias
Ercc-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ercc1em1flox/Cya mice (Catalog S-CKO-02249) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000003645
NCBI RefSeq
NM_007948
Target Region
Exon 4~6
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Ercc1, or Excision repair cross-complementation group 1, is a key gene encoding a subunit of the ERCC1-XPF nuclease. This enzyme is crucial for DNA repair, especially in the nucleotide excision repair (NER) pathway, which repairs bulky DNA adducts like intrastrand and interstrand crosslinks. ERCC1-XPF also participates in double-strand break repair, interstrand crosslink repair, base excision repair as a backup, and telomere length regulation [1,3].
In mouse models, Ercc1 deficiency (Ercc1Δ/-) leads to rapid manifestation of vascular aging characteristics. The aortas of these mice show arterial thickening, loss of cells, discontinuous endothelial layer, phenotypical switching of vascular smooth muscle cells (VSMCs), increased matrix metalloproteinase (MMP) activity, elastin fragmentation, and proteoglycan deposition. This indicates that Ercc1 plays a vital role in maintaining vascular homeostasis [2].
In conclusion, Ercc1 is essential for DNA repair processes, influencing genomic stability. The Ercc1Δ/-mouse model reveals its significance in vascular aging. Understanding Ercc1's function not only provides insights into normal biological processes but also offers potential implications for diseases related to DNA repair deficiencies and vascular aging.
References：
1. Manandhar, Mandira, Boulware, Karen S, Wood, Richard D. 2015. The ERCC1 and ERCC4 (XPF) genes and gene products. In Gene, 569, 153-61. doi:10.1016/j.gene.2015.06.026. https://pubmed.ncbi.nlm.nih.gov/26074087/
2. van der Linden, Janette, Stefens, Sanne J M, Heredia-Genestar, José María, Essers, Jeroen, van der Pluijm, Ingrid. 2024. Ercc1 DNA repair deficiency results in vascular aging characterized by VSMC phenotype switching, ECM remodeling, and an increased stress response. In Aging cell, 23, e14126. doi:10.1111/acel.14126. https://pubmed.ncbi.nlm.nih.gov/38451018/
3. Faridounnia, Maryam, Folkers, Gert E, Boelens, Rolf. 2018. Function and Interactions of ERCC1-XPF in DNA Damage Response. In Molecules (Basel, Switzerland), 23, . doi:10.3390/molecules23123205. https://pubmed.ncbi.nlm.nih.gov/30563071/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen