C57BL/6JCya-Ewsr1em1flox/Cya
Common Name
Ewsr1-flox
Product ID
S-CKO-02279
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-14030-Ewsr1-B6J-VA
When using this mouse strain in a publication, please cite “Ewsr1-flox Mouse (Catalog S-CKO-02279) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ewsr1-flox
Strain ID
CKOCMP-14030-Ewsr1-B6J-VA
Gene Name
Product ID
S-CKO-02279
Gene Alias
Ews, Ewsh
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000079949
NCBI RefSeq
NM_007968
Target Region
Exon 4
Size of Effective Region
~2.7 kb
Overview of Gene Research
Ewsr1, also known as Ewing sarcoma breakpoint region 1, is a gene with significant importance in various biological processes. It has been shown to play a role in maintaining centromere identity as it interacts with CENP-A and is required for keeping CENP-A at the centromere in interphase cells. Ewsr1 binds to CENP-A through the SYGQ2 region within the prion-like domain and to R-loops via its RNA-recognition motif, which are essential for maintaining centromere identity [2].
EWSR1 is the most frequently rearranged gene in mesenchymal neoplasia. Its chimeric oncoproteins drive a wide variety of neoplasms. For example, EWSR1 fusions with CREB family members (such as ATF1 and CREB1), as well as fusions in emerging entities like mesenchymal neoplasms with EWSR1::PATZ1 and EWSR1::NFATC2, are involved in tumorigenesis [1]. EWSR1-SMAD3 rearranged fibroblastic tumor is a recently characterized neoplasm with distinct clinicopathologic features and a recurrent EWSR1-SMAD3 gene fusion [3,4]. EWSR1::ATF1 translocation is a common tumor driver for distinct human neoplasms [5].
In conclusion, Ewsr1 is crucial for maintaining centromere identity. Its rearrangements, especially in mesenchymal neoplasia, play a significant role in tumor development. Understanding Ewsr1 through genetic models can provide insights into the mechanisms of neoplasm formation, potentially leading to new strategies for cancer treatment.
References：
1. Towery, Emily Anne, Papke, David James. 2024. EWSR1: the promiscuous king of mesenchymal neoplasia. In Journal of clinical pathology, 77, 721-725. doi:10.1136/jcp-2023-208867. https://pubmed.ncbi.nlm.nih.gov/39209444/
2. Kitagawa, Risa, Niikura, Yohei, Becker, Argentina, Houghton, Peter J, Kitagawa, Katsumi. 2023. EWSR1 maintains centromere identity. In Cell reports, 42, 112568. doi:10.1016/j.celrep.2023.112568. https://pubmed.ncbi.nlm.nih.gov/37243594/
3. Habeeb, Omar, Korty, Katelen E, Azzato, Elizabeth M, Ko, Jennifer S, Billings, Steven D. 2020. EWSR1-SMAD3 rearranged fibroblastic tumor: Case series and review. In Journal of cutaneous pathology, 48, 255-262. doi:10.1111/cup.13870. https://pubmed.ncbi.nlm.nih.gov/32901982/
4. Foot, Oliver, Hallin, Magnus, Jones, Robin L, Sumathi, Vaiyapuri P, Thway, Khin. 2020. EWSR1-SMAD3-Positive Fibroblastic Tumor. In International journal of surgical pathology, 29, 179-181. doi:10.1177/1066896920938124. https://pubmed.ncbi.nlm.nih.gov/32615834/
5. Bianco, Julia Raffaella, Li, YiJing, Petranyi, Agota, Fabian, Zsolt. 2024. EWSR1::ATF1 Translocation: A Common Tumor Driver of Distinct Human Neoplasms. In International journal of molecular sciences, 25, . doi:10.3390/ijms252413693. https://pubmed.ncbi.nlm.nih.gov/39769457/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.