C57BL/6JCya-Igf2bp1em1flox/Cya
Common Name:
Igf2bp1-flox
Product ID:
S-CKO-02291
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Igf2bp1-flox
Strain ID
CKOCMP-140486-Igf2bp1-B6J-VA
Gene Name
Product ID
S-CKO-02291
Gene Alias
CRD-BP; Crdbp; D030026A21Rik; D11Moh40e; D11Moh45; IMP1; Neilsen; ZBP-1; Zbp1; mir-3063
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Igf2bp1em1flox/Cya mice (Catalog S-CKO-02291) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000013559
NCBI RefSeq
NM_009951
Target Region
Exon 3~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Igf2bp1, insulin-like growth factor 2 mRNA-binding protein 1, is an oncofetal RNA-binding protein. Its main function is to stabilize target transcripts by protecting them from miRNA-mediated degradation. It is involved in various biological processes, especially in cancer-related pathways, and has been linked to the progression of multiple cancer types [3].
In cancer research, studies have shown diverse roles of Igf2bp1. In liver cancer, PRMT3-mediated arginine methylation of Igf2bp1 at R452 promotes oxaliplatin resistance, as it stabilizes the mRNA of HEG1, an effector of the PRMT3-Igf2bp1 axis [1]. In endometrial cancer, Igf2bp1 overexpression stabilizes PEG10 mRNA in an m6A-dependent manner, promoting cancer cell proliferation, regulating the cell cycle, and driving cancer progression [2]. In breast cancer, USP10 stabilizes Igf2bp1, which then binds to and stabilizes CPT1A mRNA, promoting breast cancer metastasis [4]. In esophageal squamous cell carcinoma, Igf2bp1 binds and stabilizes INHBA mRNA, activating the Smad2/3 signaling pathway and promoting cell invasion and migration [5]. In non-small cell lung cancer, the lncRNA MNX1-AS1 drives phase separation of Igf2bp1, increasing the stability of c-Myc and E2F1 mRNA and promoting cell-cycle progression and proliferation [6]. In bladder cancer, circFAM13B competitively binds to Igf2bp1, reducing its binding to PKM2 3'UTR, decreasing PKM2 mRNA stability, inhibiting glycolysis, and enhancing immunotherapy sensitivity [7].
In summary, Igf2bp1 plays a crucial role in multiple cancer-related biological processes. These findings from in vivo and in vitro functional studies, though not specifically from KO/CKO mouse models in the provided references, highlight its significance in cancer development, progression, and response to therapy, suggesting that Igf2bp1 could be a potential therapeutic target in various cancers.
References:
1. Shi, Yunxing, Niu, Yi, Yuan, Yichuan, Yuan, Yunfei, Li, Binkui. 2023. PRMT3-mediated arginine methylation of IGF2BP1 promotes oxaliplatin resistance in liver cancer. In Nature communications, 14, 1932. doi:10.1038/s41467-023-37542-5. https://pubmed.ncbi.nlm.nih.gov/37024475/
2. Zhang, Lin, Wan, Yicong, Zhang, Zihan, Cheng, Wenjun, Zhu, Lan. 2021. IGF2BP1 overexpression stabilizes PEG10 mRNA in an m6A-dependent manner and promotes endometrial cancer progression. In Theranostics, 11, 1100-1114. doi:10.7150/thno.49345. https://pubmed.ncbi.nlm.nih.gov/33391523/
3. Glaß, Markus, Hüttelmaier, Stefan. 2023. IGF2BP1-An Oncofetal RNA-Binding Protein Fuels Tumor Virus Propagation. In Viruses, 15, . doi:10.3390/v15071431. https://pubmed.ncbi.nlm.nih.gov/37515119/
4. Shi, Jiajun, Zhang, Qianyi, Yin, Xi, Wang, Shouyu, Zhang, Weijie. 2023. Stabilization of IGF2BP1 by USP10 promotes breast cancer metastasis via CPT1A in an m6A-dependent manner. In International journal of biological sciences, 19, 449-464. doi:10.7150/ijbs.76798. https://pubmed.ncbi.nlm.nih.gov/36632454/
5. Wang, Juan-Juan, Chen, Ding-Xiong, Zhang, Yu, Hao, Jia-Jie, Wang, Ming-Rong. 2023. Elevated expression of the RNA-binding protein IGF2BP1 enhances the mRNA stability of INHBA to promote the invasion and migration of esophageal squamous cancer cells. In Experimental hematology & oncology, 12, 75. doi:10.1186/s40164-023-00429-8. https://pubmed.ncbi.nlm.nih.gov/37644505/
6. Zhu, Qingqing, Zhang, Chongguo, Qu, Tianyu, Guo, Renhua, Zhang, Erbao. . MNX1-AS1 Promotes Phase Separation of IGF2BP1 to Drive c-Myc-Mediated Cell-Cycle Progression and Proliferation in Lung Cancer. In Cancer research, 82, 4340-4358. doi:10.1158/0008-5472.CAN-22-1289. https://pubmed.ncbi.nlm.nih.gov/36214649/
7. Lv, Jiancheng, Li, Kai, Yu, Hao, Yang, Xiao, Lu, Qiang. 2023. HNRNPL induced circFAM13B increased bladder cancer immunotherapy sensitivity via inhibiting glycolysis through IGF2BP1/PKM2 pathway. In Journal of experimental & clinical cancer research : CR, 42, 41. doi:10.1186/s13046-023-02614-3. https://pubmed.ncbi.nlm.nih.gov/36747239/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen