C57BL/6JCya-F2rem1flox/Cya
Common Name:
F2r-flox
Product ID:
S-CKO-02316
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
F2r-flox
Strain ID
CKOCMP-14062-F2r-B6J-VA
Gene Name
Product ID
S-CKO-02316
Gene Alias
Cf2r; Par1; ThrR
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-F2rem1flox/Cya mice (Catalog S-CKO-02316) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059193
NCBI RefSeq
NM_010169
Target Region
Exon 2
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
F2r, also known as Coagulation Factor II Thrombin Receptor, is an important member of the G-protein-coupled receptor (GPCR) family. It plays a role in regulating blood clotting through protein hydrolytic cleavage-mediated receptor activation. It is also involved in various signaling pathways such as the Akt and NFκB signaling pathways, and is highly expressed in osteoclasts [1,2].
In studies on osteoclasts, loss-of-function experiments using F2r-targeted short hairpin RNA (sh-F2r) lentivirus in mouse bone marrow cells (MBMs) induced osteoclasts showed that F2r knockdown led to a significant increase in osteoclast activity, number, size, promoted bone resorption and F-actin ring formation with increased osteoclast marker gene expression. In contrast, overexpression of F2r blocked osteoclast formation, maturation, and acidification, indicating that F2r negatively regulates osteoclast formation and function. The mechanism may be through inhibiting the F2r-Akt and F2r-NFκB signaling pathways in response to RANKL stimulation [1].
In conclusion, F2r has a negative regulatory function on osteoclastogenesis, mainly by inhibiting the Akt and NFκB signaling pathways. These findings from mouse model-based research provide potential new therapeutic approaches for bone diseases such as osteoporosis [1].
References:
1. Zhang, Yan, Wang, He, Zhu, Guochun, Qian, Airong, Chen, Wei. 2020. F2r negatively regulates osteoclastogenesis through inhibiting the Akt and NFκB signaling pathways. In International journal of biological sciences, 16, 1629-1639. doi:10.7150/ijbs.41867. https://pubmed.ncbi.nlm.nih.gov/32226307/
2. Wu, Xingwei, Wang, Shengnan, Wang, Chenci, Wu, Chengwei, Zhao, Zhiyong. 2024. Bioinformatics analysis identifies coagulation factor II receptor as a potential biomarker in stomach adenocarcinoma. In Scientific reports, 14, 2468. doi:10.1038/s41598-024-52397-6. https://pubmed.ncbi.nlm.nih.gov/38291086/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen