C57BL/6NCya-F5em1flox/Cya
Common Name
F5-flox
Product ID
S-CKO-02323
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-14067-F5-B6N-VA
When using this mouse strain in a publication, please cite “F5-flox Mouse (Catalog S-CKO-02323) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
F5-flox
Strain ID
CKOCMP-14067-F5-B6N-VA
Gene Name
Product ID
S-CKO-02323
Gene Alias
Cf5, Cf-5
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 1
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000086040
NCBI RefSeq
NM_007976
Target Region
Exon 6~7
Size of Effective Region
~3.3 kb
Overview of Gene Research
F5, also known as Coagulation factor V, is a key regulator of blood coagulation. It is involved in the blood coagulation pathway, playing a crucial role in maintaining hemostasis. Dysregulation of F5 can lead to bleeding or thrombotic disorders [1,2,3].
In a study, a novel heterozygous 832 base pair deletion within F5 exon 13 (F5-Atlanta) was identified in a patient with severe bleeding. This deletion affected F5 and FV expression, leading to elevated levels of total and free TFPI and a FV isoform similar to FV-short described in East Texas bleeding disorder [1].
In breast cancer cells, F5 was found to be an estrogen-responsive gene. Estrogen stimulation increased F5 messenger RNA and FV protein in ERα-positive MCF-7 cells, and high F5 levels in ER-positive breast tumors were associated with increased relapse-free survival [2]. Also, the F5 6665A>G polymorphism was associated with an increased risk of venous thromboembolism in females [3].
In conclusion, F5 is essential for blood coagulation, and its dysregulation can contribute to bleeding and thrombotic disorders. Studies on F5 gene mutations and its regulation in different diseases, such as bleeding disorders and breast cancer, help us understand its role in these pathological conditions, providing insights for diagnosis and potential treatment strategies.
References：
1. Zimowski, Karen L, Petrillo, Teodolinda, Ho, Michelle D, Camire, Rodney M, Doering, Christopher B. 2021. F5-Atlanta: A novel mutation in F5 associated with enhanced East Texas splicing and FV-short production. In Journal of thrombosis and haemostasis : JTH, 19, 1653-1665. doi:10.1111/jth.15314. https://pubmed.ncbi.nlm.nih.gov/33773040/
2. Andresen, Marianne S, Sletten, Marit, Sandset, Per Morten, Stavik, Benedicte, Tinholt, Mari. 2021. Coagulation Factor V (F5) is an Estrogen-Responsive Gene in Breast Cancer Cells. In Thrombosis and haemostasis, 122, 1288-1295. doi:10.1055/a-1707-2130. https://pubmed.ncbi.nlm.nih.gov/34826880/
3. Teofilov, Sladjana, Miljanović, Olivera, Vuckovic-Filipovic, Jelena, Djordjevic, Natasa. 2025. F5 6665A>G Polymorphism Is Associated with Increased Risk of Venous Thromboembolism in Females. In International journal of molecular sciences, 26, . doi:10.3390/ijms26062403. https://pubmed.ncbi.nlm.nih.gov/40141044/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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