C57BL/6JCya-Ptk2em1flox/Cya
Common Name
Ptk2-flox
Product ID
S-CKO-02348
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-14083-Ptk2-B6J-VA
When using this mouse strain in a publication, please cite “Ptk2-flox Mouse (Catalog S-CKO-02348) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Ptk2-flox
Strain ID
CKOCMP-14083-Ptk2-B6J-VA
Gene Name
Product ID
S-CKO-02348
Gene Alias
FAK, FRNK, Fadk, FADK 1, p125FAK
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 15
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000110036
NCBI RefSeq
NM_007982
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Overview of Gene Research
PTK2, also known as focal adhesion kinase (FAK), is a cytoplasmic protein tyrosine kinase. It is crucial in various biological processes, such as cell movement, invasion, survival, gene expression, and cancer stem cell self-renewal, and is involved in multiple signaling pathways [3].
In hepatocellular carcinoma (HCC), genetic depletion of KLF7, which upregulates PTK2, impedes HMGB1-mediated HCC progression and metastasis, indicating PTK2's role in promoting HCC metastasis [1]. In IPF, PTK2 levels are higher in lung tissues of patients, and a PTK2-associated gene signature can predict prognosis, suggesting its potential as a molecular target and prognostic biomarker [2]. In HCC, PTK2 promotes cancer stem cell traits by activating Wnt/β-catenin signaling, leading to HCC recurrence and sorafenib resistance [4]. Also, in HCC, the combination of IGF1R inhibitor and PTK2 inhibitor defactinib suppresses BACH1-mediated HCC growth and metastasis, highlighting PTK2's role in HCC growth and metastasis [5]. In another study on HCC, the combination of PTK2 inhibitor defactinib and c-MET inhibitor capmatinib significantly suppressed HCC metastasis induced by ETV1, which upregulates PTK2 [6].
In conclusion, PTK2 is a key cytoplasmic protein tyrosine kinase involved in various biological processes. Through gene-knockout or related functional studies in disease models like HCC and IPF, it has been revealed that PTK2 plays important roles in tumor progression, metastasis, and may serve as a potential therapeutic target and prognostic biomarker in these diseases.
References：
1. Feng, Weibo, Chen, Jie, Huang, Wenjie, Wu, Kaichun, Xia, Limin. 2023. HMGB1-mediated elevation of KLF7 facilitates hepatocellular carcinoma progression and metastasis through upregulating TLR4 and PTK2. In Theranostics, 13, 4042-4058. doi:10.7150/thno.84388. https://pubmed.ncbi.nlm.nih.gov/37554278/
2. Feng, Anlin, Caro, Yesenia Moreno, Gardner, Colin, Knox, Kenneth S, Wang, Ting. 2023. PTK2-associated gene signature could predict the prognosis of IPF. In Respiratory research, 24, 304. doi:10.1186/s12931-023-02582-4. https://pubmed.ncbi.nlm.nih.gov/38053045/
3. Sulzmaier, Florian J, Jean, Christine, Schlaepfer, David D. 2014. FAK in cancer: mechanistic findings and clinical applications. In Nature reviews. Cancer, 14, 598-610. doi:10.1038/nrc3792. https://pubmed.ncbi.nlm.nih.gov/25098269/
4. Fan, Zhongyi, Duan, Jingjing, Wang, Lingxiong, Jiao, Shunchang, Xu, Xiaojie. 2019. PTK2 promotes cancer stem cell traits in hepatocellular carcinoma by activating Wnt/β-catenin signaling. In Cancer letters, 450, 132-143. doi:10.1016/j.canlet.2019.02.040. https://pubmed.ncbi.nlm.nih.gov/30849480/
5. Xie, Meng, Sun, Mengyu, Ji, Xiaoyu, Tian, Dean, Xia, Limin. 2022. Overexpression of BACH1 mediated by IGF2 facilitates hepatocellular carcinoma growth and metastasis via IGF1R and PTK2. In Theranostics, 12, 1097-1116. doi:10.7150/thno.65775. https://pubmed.ncbi.nlm.nih.gov/35154476/
6. Zhang, Tongyue, Wang, Yijun, Xie, Meng, Huang, Wenjie, Xia, Limin. 2022. HGF-mediated elevation of ETV1 facilitates hepatocellular carcinoma metastasis through upregulating PTK2 and c-MET. In Journal of experimental & clinical cancer research : CR, 41, 275. doi:10.1186/s13046-022-02475-2. https://pubmed.ncbi.nlm.nih.gov/36109787/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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