C57BL/6JCya-Caln1em1flox/Cya
Common Name:
Caln1-flox
Product ID:
S-CKO-02357
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Caln1-flox
Strain ID
CKOCMP-140904-Caln1-B6J-VA
Gene Name
Product ID
S-CKO-02357
Gene Alias
9630012C17Rik; Cabp8; MNCb-0849
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Caln1em1flox/Cya mice (Catalog S-CKO-02357) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111288
NCBI RefSeq
NM_021371
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Caln1, also known as calneuron 1, is a member of the calmodulin superfamily. It encodes a neuron-specific protein mapping to chromosome 7q11, with potential roles in signal transduction due to its high homology with calmodulin. It may be significant in neuron physiology, potentially related to memory and learning [8]. It is also involved in membrane remodeling processes, interacting with proteins like APOL3 to control PI(4)P synthesis, which is crucial for infection-induced inflammation processes [4,7].
In bladder cancer, CALN1 hypomethylation is associated with advanced tumor stage, high histological grade, and is an independent risk factor for intravesical recurrence in non-muscle-invasive bladder cancer, suggesting its potential as a biomarker [1]. In schizophrenia, CALN1 has been identified as a target of miR-137, with miR-137 overexpression reducing CALN1 expression, indicating a possible role in the disease mechanism [2]. In glioma, CALN1 expression is decreased and positively correlated with prognosis, and its abnormal decrease may lead to the invasion of tumor-promoting immune cells, making it a potential prognostic marker and therapeutic target [3]. In aldosterone-producing adenoma, CALN1 overexpression increases Ca2+ in the endoplasmic reticulum and aldosterone production, and silencing CALN1 prevents aldosterone production, suggesting it as a potential therapeutic target for excess aldosterone production [5]. In metastatic osteosarcoma, exosomal miR-675 down-regulates CALN1 expression, promoting cell migration and invasion, and serum exosomal miR-675 may serve as a biomarker for metastasis [6].
In conclusion, Caln1 is involved in multiple biological processes and disease conditions. Its functions range from being potentially important in neuron-related physiological activities to playing roles in various cancers, schizophrenia, and aldosterone-related disorders. Studies on Caln1 contribute to understanding disease mechanisms and may offer new perspectives for biomarker discovery and therapeutic targeting.
References：
1. Takagi, Kimiaki, Naruse, Azumi, Akita, Kazutoshi, Horie, Masanobu, Kikuchi, Arizumi. 2022. CALN1 hypomethylation as a biomarker for high-risk bladder cancer. In BMC urology, 22, 176. doi:10.1186/s12894-022-01136-y. https://pubmed.ncbi.nlm.nih.gov/36352401/
2. Xia, Shihui, Zhou, Xinyao, Wang, Teng, He, Lin, Zhao, Xinzhi. 2015. Experimental validation of candidate schizophrenia gene CALN1 as a target for microRNA-137. In Neuroscience letters, 602, 110-4. doi:10.1016/j.neulet.2015.07.001. https://pubmed.ncbi.nlm.nih.gov/26163462/
3. Zhang, Han, Zhang, Jinhao, Wang, Tao. . [Bioinformatic analysis of the clinical significance of calneuron 1 (CALN1) in glioma and its correlation with immune cell infiltration]. In Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology, 39, 205-212. doi:. https://pubmed.ncbi.nlm.nih.gov/36946344/
4. Pays, Etienne. 2024. Apolipoprotein-L Functions in Membrane Remodeling. In Cells, 13, . doi:10.3390/cells13242115. https://pubmed.ncbi.nlm.nih.gov/39768205/
5. Kobuke, Kazuhiro, Oki, Kenji, Gomez-Sanchez, Celso E, Yoneda, Masayasu, Hattori, Noboru. 2017. Calneuron 1 Increased Ca2+ in the Endoplasmic Reticulum and Aldosterone Production in Aldosterone-Producing Adenoma. In Hypertension (Dallas, Tex. : 1979), 71, 125-133. doi:10.1161/HYPERTENSIONAHA.117.10205. https://pubmed.ncbi.nlm.nih.gov/29109191/
6. Gong, Liangzhi, Bao, Qiyuan, Hu, Chuanzhen, Zhang, Weibin, Shen, Yuhui. 2018. Exosomal miR-675 from metastatic osteosarcoma promotes cell migration and invasion by targeting CALN1. In Biochemical and biophysical research communications, 500, 170-176. doi:10.1016/j.bbrc.2018.04.016. https://pubmed.ncbi.nlm.nih.gov/29626470/
7. Pays, Etienne. 2024. The Janus-faced functions of Apolipoproteins L in membrane dynamics. In Cellular and molecular life sciences : CMLS, 81, 134. doi:10.1007/s00018-024-05180-9. https://pubmed.ncbi.nlm.nih.gov/38478101/
8. Wu, Y Q, Lin, X, Liu, C M, Jamrich, M, Shaffer, L G. . Identification of a human brain-specific gene, calneuron 1, a new member of the calmodulin superfamily. In Molecular genetics and metabolism, 72, 343-50. doi:. https://pubmed.ncbi.nlm.nih.gov/11286509/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen