C57BL/6NCya-Fbp1em1flox/Cya
Common Name:
Fbp1-flox
Product ID:
S-CKO-02372
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fbp1-flox
Strain ID
CKOCMP-14121-Fbp1-B6N-VA
Gene Name
Product ID
S-CKO-02372
Gene Alias
Fbp-2; Fbp2; Fbp3
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
13
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Fbp1em1flox/Cya mice (Catalog S-CKO-02372) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000092888
NCBI RefSeq
NM_019395
Target Region
Exon 2
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Fbp1, also known as fructose 1,6-bisphosphatase 1, is a key enzyme in gluconeogenesis. It plays a crucial role in maintaining metabolic homeostasis, with its activity influencing carbohydrate metabolism pathways. Genetic models, such as knockout (KO) and conditional knockout (CKO) mouse models, have been instrumental in studying its functions.
In liver-related research, hepatocyte-specific Fbp1 deletion in mice leads to steatosis, activation and senescence of hepatic stellate cells (HSCs), and promotes tumour progression, indicating Fbp1 as a metabolic tumour suppressor in liver cancer [1]. In Kras-driven lung cancer, loss of Fbp1 in natural killer (NK) cells causes their dysfunction by inhibiting glycolysis, showing its role in NK cell-tumour cell interactions during tumour development [2]. In keratinocytes, Fbp1 promotes differentiation and inhibits proliferation, and its deficiency in mice facilitates psoriasis-like skin lesions development [3]. In non-small cell lung cancer (NSCLC), overexpression of Fbp1 decreases the proportion of CD133-positive cells and weakens tumorigenicity by promoting the ubiquitination and degradation of Notch1 intracellular domain [4].
In conclusion, Fbp1 is essential for metabolic regulation and plays significant roles in multiple disease conditions. KO/CKO mouse models have been vital in uncovering its functions in liver cancer, lung cancer, psoriasis, and NSCLC, highlighting its potential as a therapeutic target in these diseases.
References:
1. Li, Fuming, Huangyang, Peiwei, Burrows, Michelle, Li, Bo, Simon, M Celeste. 2020. FBP1 loss disrupts liver metabolism and promotes tumorigenesis through a hepatic stellate cell senescence secretome. In Nature cell biology, 22, 728-739. doi:10.1038/s41556-020-0511-2. https://pubmed.ncbi.nlm.nih.gov/32367049/
2. Cong, Jingjing, Wang, Xianwei, Zheng, Xiaohu, Tian, Zhigang, Wei, Haiming. 2018. Dysfunction of Natural Killer Cells by FBP1-Induced Inhibition of Glycolysis during Lung Cancer Progression. In Cell metabolism, 28, 243-255.e5. doi:10.1016/j.cmet.2018.06.021. https://pubmed.ncbi.nlm.nih.gov/30033198/
3. Zhang, Pengfei, Yang, Ju, Liu, Xiong, Zhou, Wei, Gao, Yue. 2024. FBP1 orchestrates keratinocyte proliferation/differentiation and suppresses psoriasis through metabolic control of histone acetylation. In Cell death & disease, 15, 392. doi:10.1038/s41419-024-06706-6. https://pubmed.ncbi.nlm.nih.gov/38834617/
4. He, Tianyu, Wang, Yanye, Lv, Wang, Shen, Han-Ming, Hu, Jian. 2024. FBP1 inhibits NSCLC stemness by promoting ubiquitination of Notch1 intracellular domain and accelerating degradation. In Cellular and molecular life sciences : CMLS, 81, 87. doi:10.1007/s00018-024-05138-x. https://pubmed.ncbi.nlm.nih.gov/38349431/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen