C57BL/6JCya-Fdft1em1flox/Cya
Common Name:
Fdft1-flox
Product ID:
S-CKO-02384
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fdft1-flox
Strain ID
CKOCMP-14137-Fdft1-B6J-VA
Gene Name
Product ID
S-CKO-02384
Gene Alias
SQS; SS
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fdft1em1flox/Cya mice (Catalog S-CKO-02384) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000224625
NCBI RefSeq
NM_010191
Target Region
Exon 5
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Fdft1, also known as Farnesyl-Diphosphate Farnesyltransferase 1, is a key gene in the cholesterol synthesis pathway, with Squalene synthase (SQS) encoded by it being a crucial regulator in this process [4,6]. Cholesterol synthesis is essential for cell membrane integrity, steroid hormone production, and bile acid synthesis, highlighting the overall biological importance of Fdft1. Genetic models are valuable for studying its functions.
In colorectal cancer, fasting upregulates Fdft1, which then inhibits aerobic glycolysis and proliferation by suppressing the AKT/mTOR/HIF1α pathway, indicating its role as a tumor suppressor [1]. In contrast, in tongue squamous cell carcinoma, Fdft1 acts as an oncogene, promoting oncogenesis, and its repression by piR-39980 can prevent oncogenesis through regulating proliferation and apoptosis via hypoxia [2]. In clear cell renal cell carcinoma, upregulated Fdft1 inhibits cell proliferation, migration, and invasion potentially via the AKT signaling pathway [3]. In glioblastoma stem cells, Fdft1 is transcriptionally regulated by SREBP2 and activates the AKT pathway to maintain stemness, and its inhibition can be a strategy to eliminate these cells [5].
In conclusion, Fdft1 is essential in cholesterol synthesis and plays diverse roles in different disease conditions. Studies using genetic models, such as potentially KO/CKO mouse models (not explicitly mentioned in all references but inferred as valuable), have revealed its significance in cancer-related biological processes like cell proliferation, migration, and stemness maintenance, providing insights into potential therapeutic strategies for cancers like colorectal, tongue squamous cell, clear cell renal cell carcinomas, and glioblastoma.
References:
1. Weng, Mei-Lin, Chen, Wan-Kun, Chen, Xiang-Yuan, Zhang, Xiao-Ping, Miao, Chang-Hong. 2020. Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression. In Nature communications, 11, 1869. doi:10.1038/s41467-020-15795-8. https://pubmed.ncbi.nlm.nih.gov/32313017/
2. Chattopadhyay, Trisha, Mallick, Bibekanand. 2023. FDFT1 repression by piR-39980 prevents oncogenesis by regulating proliferation and apoptosis through hypoxia in tongue squamous cell carcinoma. In Life sciences, 329, 121954. doi:10.1016/j.lfs.2023.121954. https://pubmed.ncbi.nlm.nih.gov/37473805/
3. Huang, Ruizhen, Zhang, Chiyu, Wang, Xing, Lin, Tao, Hu, Honglin. 2022. Identification of FDFT1 as a potential biomarker associated with ferroptosis in ccRCC. In Cancer medicine, 11, 3993-4004. doi:10.1002/cam4.4716. https://pubmed.ncbi.nlm.nih.gov/35322581/
4. Chen, Wujun, Xu, Jiazhen, Wu, Yudong, Shao, Yingchun, Xing, Dongming. 2023. The potential role and mechanism of circRNA/miRNA axis in cholesterol synthesis. In International journal of biological sciences, 19, 2879-2896. doi:10.7150/ijbs.84994. https://pubmed.ncbi.nlm.nih.gov/37324939/
5. Mo, Hui, Shao, Jiajia, Li, Zhun, Wang, Peng, Liao, Jianwei. 2024. FDFT1 maintains glioblastoma stem cells through activation of the Akt pathway. In Stem cell research & therapy, 15, 492. doi:10.1186/s13287-024-04102-7. https://pubmed.ncbi.nlm.nih.gov/39707501/
6. Dong, Xi, Zhu, Yue, Wang, Shan, Sun, Guibo, Sun, Xiaobo. 2020. Bavachinin inhibits cholesterol synthesis enzyme FDFT1 expression via AKT/mTOR/SREBP-2 pathway. In International immunopharmacology, 88, 106865. doi:10.1016/j.intimp.2020.106865. https://pubmed.ncbi.nlm.nih.gov/32827918/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen