C57BL/6JCya-Fgfr3em1flox/Cya
Common Name:
Fgfr3-flox
Product ID:
S-CKO-02416
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Fgfr3-flox
Strain ID
CKOCMP-14184-Fgfr3-B6J-VA
Gene Name
Product ID
S-CKO-02416
Gene Alias
CD333; FR3; Fgfr-3; Flg-2; HBGFR; Mfr3; sam3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fgfr3em1flox/Cya mice (Catalog S-CKO-02416) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169212
NCBI RefSeq
NM_001163215
Target Region
Exon 5~7
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Fgfr3, fibroblast growth factor receptor 3, is a transmembrane kinase protein. It functions in regulating bone growth, expressed in chondrocytes and mature osteoblasts [3]. The FGFR3-related signaling pathway is involved in multiple biological processes, and its dysregulation is associated with various diseases, highlighting its biological importance [4]. Genetic models, like KO/CKO mouse models, are valuable for studying its functions.
Mutations in Fgfr3 cause achondroplasia, the most common form of dwarfism in humans, and related chondrodysplasia syndromes [3]. These mutations increase signaling through multiple mechanisms, paradoxically suppressing growth plate chondrocyte proliferation and maturation, leading to decreased bone elongation [3]. FGFR3-TACC3 fusion, prevalent in 3-4% of human glioblastoma, activates FGFR3 kinase signaling, promoting cell proliferation and tumor progression [1]. FGFR3 mutations occur in up to half of bladder cancers, playing a role in tumorigenesis and influencing responses to immune checkpoint inhibitors [2]. FGFR3 overexpression in metastatic colorectal cancer is associated with an unfavorable prognosis [5]. In ovarian cancer, FGFR3 overexpression promotes cisplatin-resistance by phosphorylating EGFR and activating the PI3K/AKT pathway [6].
In conclusion, Fgfr3 is crucial for bone growth regulation. Studies using KO/CKO mouse models could potentially further clarify its role in bone-related diseases like achondroplasia. Its dysregulation also significantly contributes to various cancers, such as glioblastoma, bladder, colorectal, and ovarian cancers, making it an important target for potential therapeutic interventions.
References:
1. Gött, Hanna, Uhl, Eberhard. 2022. FGFR3-TACCs3 Fusions and Their Clinical Relevance in Human Glioblastoma. In International journal of molecular sciences, 23, . doi:10.3390/ijms23158675. https://pubmed.ncbi.nlm.nih.gov/35955806/
2. Noeraparast, Maxim, Krajina, Katarina, Pichler, Renate, Ahyai, Sascha, Pichler, Martin. 2024. FGFR3 alterations in bladder cancer: Sensitivity and resistance to targeted therapies. In Cancer communications (London, England), 44, 1189-1208. doi:10.1002/cac2.12602. https://pubmed.ncbi.nlm.nih.gov/39161208/
3. Ornitz, David M, Legeai-Mallet, Laurence. 2017. Achondroplasia: Development, pathogenesis, and therapy. In Developmental dynamics : an official publication of the American Association of Anatomists, 246, 291-309. doi:10.1002/dvdy.24479. https://pubmed.ncbi.nlm.nih.gov/27987249/
4. Costa, Ricardo, Carneiro, Benedito A, Taxter, Timothy, Chae, Young Kwang, Giles, Francis J. . FGFR3-TACC3 fusion in solid tumors: mini review. In Oncotarget, 7, 55924-55938. doi:10.18632/oncotarget.10482. https://pubmed.ncbi.nlm.nih.gov/27409839/
5. Fromme, J E, Schildhaus, H-U. . [FGFR3 overexpression is a relevant alteration in colorectal cancer]. In Der Pathologe, 39, 189-192. doi:10.1007/s00292-018-0504-0. https://pubmed.ncbi.nlm.nih.gov/30267148/
6. Zhao, Jing, Tan, Wenxi, Zhang, Lingyi, Cui, Manhua, Zhao, Shuhua. 2021. FGFR3 phosphorylates EGFR to promote cisplatin-resistance in ovarian cancer. In Biochemical pharmacology, 190, 114536. doi:10.1016/j.bcp.2021.114536. https://pubmed.ncbi.nlm.nih.gov/33794187/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen