C57BL/6JCya-Fgl2em1flox/Cya
Common Name:
Fgl2-flox
Product ID:
S-CKO-02420
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fgl2-flox
Strain ID
CKOCMP-14190-Fgl2-B6J-VA
Gene Name
Product ID
S-CKO-02420
Gene Alias
musfiblp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fgl2em1flox/Cya mice (Catalog S-CKO-02420) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035799
NCBI RefSeq
NM_008013
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Fgl2, a member of the thrombospondin family and fibrinogen-related proteins family, is a multifunctional protein. It is known for modulating prothrombin activity, inducing immune tolerance, and regulating the activity of immune cells and tumor cells. It is involved in various pathways such as those related to coagulation, inflammation, and immune-regulation, which are crucial in many biological processes and disease conditions [1,2,4]. Genetic models like gene knockout (KO) mouse models are valuable for studying its functions.
In melanoma and ovarian cancer models, Fgl2 knockout mice demonstrated that absence of Fgl2 led to a more activated tumor microenvironment (TME), with activated dendritic cells, T cells, and natural killer cells, and prolonged survival in the B16F10 melanoma model, and synergized with oncolytic virus in the ID8-p53-/-Brca2-/-ovarian cancer model, indicating Fgl2 promotes tumour growth and attenuates infiltration of activated immune cells [5]. In glioblastoma, T cells expressing Fgl2-specific single-chain variable fragments can induce tumor-specific CD8+ tissue-resident memory T (TRM) cells that prevent glioblastoma recurrence, suggesting a role in anti-glioblastoma immunity [3]. Also, Fgl2 deficiency alleviated maternal inflammation-induced blood-brain barrier damage by blocking PI3K/NF-κB mediated endothelial oxidative stress in a mouse model of maternal inflammation [6].
In conclusion, Fgl2 is crucial in regulating immune responses, tumour growth, and inflammation-related processes. KO mouse models have been instrumental in revealing its role in cancer, such as melanoma, ovarian cancer, and glioblastoma, as well as in conditions like maternal-inflammation-induced brain damage. Understanding Fgl2's functions provides potential therapeutic targets for these diseases.
References:
1. Ma, Xiaoyu, Zhu, Hongtao, Cheng, Lidong, Shu, Kai, Zhang, Suojun. 2022. Targeting FGL2 in glioma immunosuppression and malignant progression. In Frontiers in oncology, 12, 1004700. doi:10.3389/fonc.2022.1004700. https://pubmed.ncbi.nlm.nih.gov/36313679/
2. Chen, Jiongming, Wu, Lei, Li, Yongsheng. 2024. FGL1 and FGL2: emerging regulators of liver health and disease. In Biomarker research, 12, 53. doi:10.1186/s40364-024-00601-0. https://pubmed.ncbi.nlm.nih.gov/38816776/
3. Zhao, Qingnan, Hu, Jiemiao, Kong, Lingyuan, Heimberger, Amy B, Li, Shulin. 2023. FGL2-targeting T cells exhibit antitumor effects on glioblastoma and recruit tumor-specific brain-resident memory T cells. In Nature communications, 14, 735. doi:10.1038/s41467-023-36430-2. https://pubmed.ncbi.nlm.nih.gov/36759517/
4. Wang, Chaoyang, Sun, He, Wang, Rui, Ma, Xiaochun, Sun, Yini. 2024. FGL2: A new target molecule for coagulation and immune regulation in infectious disease. In International immunopharmacology, 143, 113505. doi:10.1016/j.intimp.2024.113505. https://pubmed.ncbi.nlm.nih.gov/39488038/
5. Galpin, Kristianne J C, Rodriguez, Galaxia M, Maranda, Vincent, Ardolino, Michele, Vanderhyden, Barbara C. 2024. FGL2 promotes tumour growth and attenuates infiltration of activated immune cells in melanoma and ovarian cancer models. In Scientific reports, 14, 787. doi:10.1038/s41598-024-51217-1. https://pubmed.ncbi.nlm.nih.gov/38191799/
6. Huang, Lianjing, Zhan, Di, Xing, Ying, Zhang, Cai, Luo, Xiaoping. 2023. FGL2 deficiency alleviates maternal inflammation-induced blood-brain barrier damage by blocking PI3K/NF-κB mediated endothelial oxidative stress. In Frontiers in immunology, 14, 1157027. doi:10.3389/fimmu.2023.1157027. https://pubmed.ncbi.nlm.nih.gov/37051251/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen