C57BL/6JCya-Fh1em1flox/Cya
Common Name:
Fh1-flox
Product ID:
S-CKO-02422
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fh1-flox
Strain ID
CKOCMP-14194-Fh1-B6J-VA
Gene Name
Product ID
S-CKO-02422
Gene Alias
Fh; Fh-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fh1em1flox/Cya mice (Catalog S-CKO-02422) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027810
NCBI RefSeq
NM_010209
Target Region
Exon 6
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Fh1, or fumarate hydratase 1, is a gene with diverse functions. In the context of mammalian biology, it has been associated with the control of Atf4 expression [4]. In plants, the Arabidopsis thaliana FH1 (At3g25500) is a Class I membrane-anchored formin that contributes to actin dynamics, microtubule stability, and is involved in cell morphogenesis and organ development [2,3,5].
In a mouse model of non-alcoholic steatohepatitis (NASH), the Klf10-Fh1 axis was found to be crucial. Hepatocyte-specific knockout of Klf10 (Klf10LKO) increased lipid accumulation, cell death, inflammation, and fibrosis in NASH-diet-fed mice and reduced the protective effects of treadmill exercise against NASH. Klf10 promotes the expression of Fh1, which in turn reduces fumarate accumulation in hepatocytes. This decreases the trimethyl (me3) levels of histone 3 lysine 4 (H3K4me3) on lipogenic genes promoters to attenuate lipogenesis, thus ameliorating NASH-related symptoms [1].
In summary, Fh1 plays important roles in both mammalian and plant biological processes. In mice, the Fh1-related Klf10-Fh1 axis is significant in the context of NASH, where its manipulation through gene knockout models has revealed its role in regulating lipid metabolism, inflammation, and fibrosis, providing insights into potential therapeutic strategies for NASH.
References:
1. Luo, Hong-Yang, Mu, Wang-Jing, Chen, Min, Chen, Hu-Min, Guo, Liang. 2024. Hepatic Klf10-Fh1 axis promotes exercise-mediated amelioration of NASH in mice. In Metabolism: clinical and experimental, 155, 155916. doi:10.1016/j.metabol.2024.155916. https://pubmed.ncbi.nlm.nih.gov/38615945/
2. Rosero, Amparo, Oulehlová, Denisa, Stillerová, Lenka, Žárský, Viktor, Cvrčková, Fatima. 2016. Arabidopsis FH1 Formin Affects Cotyledon Pavement Cell Shape by Modulating Cytoskeleton Dynamics. In Plant & cell physiology, 57, 488-504. doi:10.1093/pcp/pcv209. https://pubmed.ncbi.nlm.nih.gov/26738547/
3. Oulehlová, Denisa, Kollárová, Eva, Cifrová, Petra, Žárský, Viktor, Cvrčková, Fatima. . Arabidopsis Class I Formin FH1 Relocates between Membrane Compartments during Root Cell Ontogeny and Associates with Plasmodesmata. In Plant & cell physiology, 60, 1855-1870. doi:10.1093/pcp/pcz102. https://pubmed.ncbi.nlm.nih.gov/31135031/
4. Quirós, Pedro M, Prado, Miguel A, Zamboni, Nicola, Gygi, Steven P, Auwerx, Johan. 2017. Multi-omics analysis identifies ATF4 as a key regulator of the mitochondrial stress response in mammals. In The Journal of cell biology, 216, 2027-2045. doi:10.1083/jcb.201702058. https://pubmed.ncbi.nlm.nih.gov/28566324/
5. Cifrová, Petra, Oulehlová, Denisa, Kollárová, Eva, Schwarzerová, Kateřina, Cvrčková, Fatima. 2020. Division of Labor Between Two Actin Nucleators-the Formin FH1 and the ARP2/3 Complex-in Arabidopsis Epidermal Cell Morphogenesis. In Frontiers in plant science, 11, 148. doi:10.3389/fpls.2020.00148. https://pubmed.ncbi.nlm.nih.gov/32194585/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen