C57BL/6JCya-Zcchc14em1flox/Cya
Common Name:
Zcchc14-flox
Product ID:
S-CKO-02467
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zcchc14-flox
Strain ID
CKOCMP-142682-Zcchc14-B6J-VA
Gene Name
Product ID
S-CKO-02467
Gene Alias
BDG29
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zcchc14em1flox/Cya mice (Catalog S-CKO-02467) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046386
NCBI RefSeq
NM_080855
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Zcchc14, a CCHC-type zinc finger protein, is expressed in human and mouse tissues. It has been implicated in various biological processes and diseases. It is involved in pathways such as MAPK-P38, and in complex with non-canonical TENT4 poly(A) polymerases, it is essential for the replication of certain viruses [1,2,3,5].
In non-small cell lung cancer (NSCLC), knockdown of Zcchc14 using Nuclease technology KO plasmids enhanced cancer cell proliferation and invasion, and up-regulated p-P38, cyclinD1 and MMP7, suggesting Zcchc14 regulates NSCLC development via the P38 pathway [1].
In hepatitis A virus (HAV) replication, Zcchc14 binds to a stem-loop in the HAV 5' untranslated RNA, recruits TENT4, and is required for viral RNA synthesis. Chemical inhibition of TENT4A/B affected viral RNA synthesis but not the HAV 3' poly(A) tail length [2].
In hepatocellular carcinoma (HCC), knockdown of Zcchc14 inactivated the Akt/GSK3β/β-catenin signaling and inhibited HCC cell proliferation, while overexpression of Zcchc14 had the opposite effect [4].
In conclusion, Zcchc14 plays crucial regulatory roles in multiple disease conditions, including NSCLC, HCC, and viral infections such as HAV. Gene knockout studies have been instrumental in revealing its functions in these diseases, highlighting its potential as a therapeutic target.
References：
1. Shi, Xiuying, Han, Xu, Cao, Yu, Li, Cheng, Cao, Yaming. 2020. ZCCHC14 regulates proliferation and invasion of non-small cell lung cancer through the MAPK-P38 signalling pathway. In Journal of cellular and molecular medicine, 25, 1406-1414. doi:10.1111/jcmm.16223. https://pubmed.ncbi.nlm.nih.gov/33345444/
2. Li, You, Misumi, Ichiro, Shiota, Tomoyuki, Whitmire, Jason K, Lemon, Stanley M. 2022. The ZCCHC14/TENT4 complex is required for hepatitis A virus RNA synthesis. In Proceedings of the National Academy of Sciences of the United States of America, 119, e2204511119. doi:10.1073/pnas.2204511119. https://pubmed.ncbi.nlm.nih.gov/35867748/
3. Kim, Dongwan, Lee, Young-Suk, Jung, Soo-Jin, Ahn, Kwangseok, Kim, V Narry. 2020. Viral hijacking of the TENT4-ZCCHC14 complex protects viral RNAs via mixed tailing. In Nature structural & molecular biology, 27, 581-588. doi:10.1038/s41594-020-0427-3. https://pubmed.ncbi.nlm.nih.gov/32451488/
4. Cui, Yujun, Zhang, Jijun, Chen, Chiyi, Yan, Hao, Jiang, Wentao. . The Akt/GSK3β/β-catenin signaling regulated by ZCCHC14 is responsible for accelerating the proliferation of hepatocellular carcinoma. In Journal of B.U.ON. : official journal of the Balkan Union of Oncology, 26, 1958-1963. doi:. https://pubmed.ncbi.nlm.nih.gov/34761605/
5. Li, You, Lemon, Stanley M. 2024. Biochemical analysis of the host factor activity of ZCCHC14 in hepatitis A virus replication. In Journal of virology, 98, e0005724. doi:10.1128/jvi.00057-24. https://pubmed.ncbi.nlm.nih.gov/38501662/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen